Biotechnology Letters

, Volume 41, Issue 4–5, pp 613–623 | Cite as

Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana

  • Yun-Feng Bi
  • Xi-Zhu Wang
  • Shan Jiang
  • Jing-Sheng LiuEmail author
  • Ming-Zhu Zheng
  • Ping ChenEmail author
Original Research Paper



To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.


Recombinant β-glucosidase was purified, which molecular weight was about 79.5 kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2 mg ml−1 ginsenoside, 25 U ml−1 enzyme, 85 °C, and pH 5.0. β-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3 h under the given conditions, respectively. The enzyme created 1.66 mg ml−1 Rg2 from Re with 553 mg l−1 h−1, 0.85 mg ml−1, and 1.01 mg ml−1 APPT from Rg1 and Rf with 283 and 316 mg l−1 h−1 APPT.


β-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.


Biotransformation Escherichia coli Enzymatic Genes Transformation 


Supporting information

Supplementary Table 1—Strains and plasmid used.


This study was funded by Changbai Mountain Scholars Fund Project (Grant Number 00566).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10529_2019_2665_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 34 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Food Science and EngineeringJilin Agriculture UniversityChangchunChina

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