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Biotechnology Letters

, Volume 40, Issue 9–10, pp 1407–1418 | Cite as

Purification, characterization, and biocatalytic potential of a novel dextranase from Chaetomium globosum

  • Liu Yang
  • Nandi Zhou
  • Yaping Tian
Original Research Paper
  • 100 Downloads

Abstract

Objective

We aimed to identify new high-yield dextranase strains and study the catalytic potential of dextranase from the strain in industrial applications.

Results

Dextranase-producing strains were screened from soil samples, and a potential strain was identified as Chaetomium globosum according to its phenotype, biochemical characteristics, and rDNA analysis. Crude dextranase was purified to reach 10.97-fold specific activity and 18.7% recovery. The molecular weight of the enzyme was 53 kDa with an optimum temperature and pH of 60 °C and 5.5, respectively. Enzyme activity was stable at pH 4.0–7.0 and displayed sufficient thermal stability at temperatures < 50 °C. Mn2+ (10 mM) enhanced dextranase activity by 134.44%. The enzyme was identified as an endodextranase. It displayed very high hydrolytic affinity toward high-molecular weight dextran T2000, reaching 97.9% hydrolysis within 15 min at 2 U/mL.

Conclusion

Collectively, these results suggest that Chaetomium globosum shows higher production and specificity of dextranase than that from other reported strains. These findings may offer new insights into the potential of dextranase in the sugar, medical, and food industries.

Graphical abstract

Keywords

Dextranase Chaetomium globosum Dextran Hydrolysis 

Notes

Acknowledgements

This work was supported by the National Science Foundation (No. 31601558), the Fundamental Research Funds for the Central Universities (JUSRP51402A).

Supporting information

Supplementary Fig. 1—Kinetic study of Chaetomium globosum dextranase. (a) Dextran T20 as the substrate. (b) Dextran T40 as the substrate. (c) Dextran T2000 as the substrate.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Research involving human participants or animals

The authors declare that there are no studies conducted with human participants or animals.

Supplementary material

10529_2018_2599_MOESM1_ESM.doc (100 kb)
Supplementary material 1 (DOC 99 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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