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Biotechnology Letters

, Volume 40, Issue 9–10, pp 1335–1341 | Cite as

Enhancing the production of phenolic compounds during barley germination by using chitooligosaccharides to improve the antioxidant capacity of malt

  • Xiaoyu Guo
  • Zhimin Yu
  • Meihui Zhang
  • Wenzhu Tang
  • Yumei Sun
  • Xianzhen Li
Original Research Paper

Abstract

Objective

To enhance the production of phenolic compounds during barley germination using chitooligosaccharide as an elicitor to improve the antioxidant capacity of malt.

Results

When used as an elicitor for barley germination, chitooligosaccharide with a molecular weight of 3 kDa, added at 10 mg/kg barley kernels during the first steeping cycle, led to the maximum production of phenolic compounds. Compared with the control with no chitooligosaccharide added to the steeping water, the total phenolic content was increased by 54.8%. Increases in the total phenolic content of the barley malt occurred when chitooligosaccharide was applied during the first or both the first and the second steeping cycles. Thus the antioxidant capacity of barley malt was increased significantly by adding chitooligosaccharide during the steeping process.

Conclusion

Applying chitooligosaccharides during the steeping process increased the content of phenolic compounds thus improving the antioxidant capacity of the barley malt.

Keywords

Antioxidant capacity Barley Chitooligosaccharide Elicitor Malt Phenolic compound 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31771907) and the Creative and Innovative Project for High-level Researchers of Dalian (2016RQ059).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Aron PM, Shellammer TH (2010) A discussion of polyphenols in beer physical and flavor stability. J Inst Brew 12:46–50Google Scholar
  2. Dabina-Bicka I, Karklina D, Kruma Z, Dimins F (2013) Bioactive compounds in latvian beer. Proc Latv Univ Agr 30:35–42Google Scholar
  3. Fajardo J, Waniska R, Cuero R, Pettit R (1995) Phenolic compounds in peanut seeds: enhanced elicitation by chitosan and effects on growth and aflatoxin B1 production by Aspergillus flavus. Food Biotech 9:59–78CrossRefGoogle Scholar
  4. Guido LF (2016) Sulfites in beer: reviewing regulation, analysis and role. Sci Agric 73:189–197CrossRefGoogle Scholar
  5. Hameed A, Sheikh MA, Hameed A, Farooq T, Basra SMA, Jamil A (2013) Chitosan priming enhances the seed germination, antioxidants, hydrolytic enzymes, soluble proteins and sugars in wheat seeds. Agrochimica 67:32–46Google Scholar
  6. Lan W, Wang W, Yu Z, Qin Y, Luan J, Li X (2016) Enhanced germination of barley (Hordeum vulgare L.) using chitooligosaccharides as an elicitor in seed priming to improve malt quality. Biotechnol Lett 38:1935–1940CrossRefPubMedGoogle Scholar
  7. Leitao C, Marchioni E, Bergaentzlé M, Zhao M, Didierjean L, Miesch L, Holder E, Miesch M, Ennahar S (2012) Fate of polyphenols and antioxidant activity of barley throughout malting and brewing. J Cereal Sci 55:318–322CrossRefGoogle Scholar
  8. Li H, Zhao M, Cui C, Sun W, Zhao H (2016) Antioxidant activity and typical ageing compounds: their evolutions and relationships during the storage of lager beers. Int J Food Sci Tech 51:2026–2033CrossRefGoogle Scholar
  9. Lodhi G, Kim YS, Hwang JW, Kim SK, Jeon YJ, Je JY, Ahn CB, Moon SH, Jeon BT, Park PJ (2014) Chitooligosaccharide and its derivatives: preparation and biological applications. Biomed Res Int 2014:654913PubMedPubMedCentralGoogle Scholar
  10. Lu J, Zhao H, Chen J, Fan W, Dong J, Kong W, Sun J, Cao Y, Cai G (2007) Evolution of phenolic compounds and antioxidant activity during malting. J Agric Food Chem 55:10994–11001CrossRefPubMedGoogle Scholar
  11. Moreira M, Morais S, Carvalho D, Barros A, Delerue-Matos C, Guido L (2013) Brewer’s spent grain from different types of malt: evaluation of the antioxidant activity and identification of the major phenolic compounds. Food Res Int 54:382–388CrossRefGoogle Scholar
  12. Ondrejovič M, Maliar T, Polívka L, Šilhár S (2009) Polyfenoly jabĺk. Chem Listy 103:394–400Google Scholar
  13. Podio N, Baroni M, Wunderlin D (2017) Relation between polyphenol profile and antioxidant capacity of different argentinean wheat varieties. Food Chem 232:79–88CrossRefPubMedGoogle Scholar
  14. Samaras TS, Camburn PA, Chandra SX, Gordon MH, Ames JM (2005) Antioxidant properties of kined and roasted malts. J Agric Food Chem 53:8068–8074CrossRefPubMedGoogle Scholar
  15. Sharma P, Gujral H (2010) Antioxidant and polyphenol oxidase activity of germinated barley and its milling fractions. Food Chem 120:673–678CrossRefGoogle Scholar
  16. Yang Q, Pan X, Kong W, Yang H, Su Y, Zhang L, Zhang Y, Yang Y, Lan D, Liu G (2010) Antioxidant activities of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo. Food Chem 118:84–89CrossRefGoogle Scholar
  17. Yang F, Luan B, Sun Z, Yang C, Yu Z, Li X (2016) Application of chitooligosaccharides as antioxidants in beer to improve the flavour stability by protecting against beer staling during storage. Biotechnol Lett 39:305–310CrossRefPubMedGoogle Scholar
  18. Yarullina LG, Kasimova RI, Akhatova AR (2014) Activity of protective proteins in wheat plants treated with chitooligosaccharides with different degree of acetylation and infection with Bipolaris sorokiniana. Appl Biochem Biotech 50:524–530Google Scholar
  19. Yin H, Fretté XC, Christensen LP, Grevsen K (2012) Chitosan oligosaccharides promote the content of polyphenols in greek oregano (Origanum vulgare ssp. hirtum). J Agric Food Chem 60:136–143CrossRefPubMedGoogle Scholar
  20. Yin H, Du Y, Dong Z (2016) Chitin oligosaccharide and chitosan oligosaccharide: two similar but different plant elicitors. Front Plant Sci 7:522PubMedPubMedCentralGoogle Scholar
  21. Zhao H, Fan W, Dong J, Lu J, Chen J, Shan L, Lin Y, Kong W (2008) Evaluation of antioxidant activities and total phenolic content of typical malting barley varieties. Food Chem 107:296–304CrossRefGoogle Scholar
  22. Zhao H, Chen W, Lu J, Zhao M (2010) Phenolic profiles and antioxidant activities of commercial beer. Food Chem 119:1150–1158CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Biological EngineeringDalian Polytechnic UniversityDalianPeople’s Republic of China

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