Biotechnology Letters

, Volume 40, Issue 6, pp 941–948 | Cite as

Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44

  • Sen Miao
  • Hao Wu
  • Yue Zhao
  • Qinggele Caiyin
  • Yanni Li
  • Jianjun QiaoEmail author
Original Research Paper



To engineer a small nonconding RNA anti41 to enhance nisin yield by inhibiting the expression of glnR in Lactococcus lactis F44.


We constructed a screening library to determine appropriate artificial sRNAs and obtained a sRNA anti41 that can produce approximately three fold of the inhibitory effect on GlnR. Moreover, the transcription levels of the direct inhibitory targets of GlnR (glnP, glnQ, amtB, and glnK) were dramatically upregulated in the anti41 overexpression strain (F44-anti41), thereby confirming the inhibitory effect of anti41 on GlnR. In addition, anti41 overexpression improved the survival rate of cells by approximately three fold under acid stress, promoted cell growth, and increased nisin yield by 29.83%.


We were able to provide a novel strategy for the construction of robust high-producing industrial strains.


Lactococcus lactis Artificial sRNA GlnR Acid resistance Nisin yield 



This study was supported by the National Key R&D Program of China (2017YFD0201405), the National Natural Science Foundation of China (31270142, 31770076, and 31570089), and the Funds for Creative Research Groups of China (21621004). Dr. Jianjun Qiao was supported by The New Century Outstanding Talent Support Program, Education Ministry of China.

Supporting information

Table S1—Oligonucleotides used in this work.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Supplementary material

10529_2018_2550_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Sen Miao
    • 1
    • 2
  • Hao Wu
    • 1
    • 2
  • Yue Zhao
    • 1
    • 2
  • Qinggele Caiyin
    • 1
    • 2
  • Yanni Li
    • 1
    • 2
  • Jianjun Qiao
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Pharmaceutical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinPeople’s Republic of China
  2. 2.Key Laboratory of Systems Bioengineering (Ministry of Education)Tianjin UniversityTianjinPeople’s Republic of China
  3. 3.SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)TianjinPeople’s Republic of China

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