Modeling and simulation of enzymatic gluconic acid production using immobilized enzyme and CSTR–PFTR circulation reaction system
- 228 Downloads
Production of gluconic acid by using immobilized enzyme and continuous stirred tank reactor-plug flow tubular reactor (CSTR–PFTR) circulation reaction system.
A production system is constructed for gluconic acid production, which consists of a continuous stirred tank reactor (CSTR) for pH control and liquid storage and a plug flow tubular reactor (PFTR) filled with immobilized glucose oxidase (GOD) for gluconic acid production. Mathematical model is developed for this production system and simulation is made for the enzymatic reaction process. The pH inhibition effect on GOD is modeled by using a bell-type curve.
Gluconic acid can be efficiently produced by using the reaction system and the mathematical model developed for this system can simulate and predict the process well.
KeywordsGluconic acid Immobilized glucose oxidase Modeling and simulation pH CSTR PFTR
This work was supported by Shandong Province Science and Technology Development Project (2015GSF121016), the National Natural Science Foundation (61672329, 61602283), Shandong Natural Science Foundation (ZR2016FB10), and the State Key Laboratory of Microbial Technology Foundation of People’s Republic of China.
Supplementary 1.zip is the program of the simulation software.
CL, JL, and JL designed this study; CL and JL conducted the experiments; CL, JL, LG, HL, and JL analyzed the data; CL and JL wrote the paper. All the authors have read the manuscript critically.
Compliance with ethical standards
Conflict of interest
All the authors declare that there is no conflict of interest.
The authors declare that there are no studies conducted with human participants or animals.
- Gao L, Ren Y, Ma Y, Lin J, Lin J (2011) Modeling and simulation of production of metallothionein and red fluorescent fusion protein by recombinant Escherichia Coli using graphical programming. In: Riccardo DA (ed) Modeling, programming and simulations using LabVIEW Software. Intech Press, Rijeka, pp 91–106Google Scholar
- Guo Q, Liu G, Dong N, Lin J, Lin J (2013) Model predictive control of glucose feeding for fed-batch Candida utilis biomass production. Res J Biotechnol 8:3–7Google Scholar
- Lin J, Lee SM, Koo YM (2004) Model development for lactic acid fermentation and parameter optimization using genetic algorithm. J Microbiol Biotechnol 14:26–27Google Scholar
- Lin Y, Liu G, Lin H, Gao L, Lin J (2013) Analysis of batch and repeated fedbatch productions of Candida utilis cell mass using mathematical modeling method. Electron J Biotechnol 16:6–8Google Scholar
- Lin J, Gao L, Lin H, Ren Y, Lin Y, Lin J (2017) Computer simulation of bioprocess. In: Cvetković D (ed) Computer simulation. Intech Press, Rijeka (in press) Google Scholar
- Milsom PE, Meers JL (1985) Gluconic acid, itaconic acid. In: Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology, 3rd edn. Pergamon, Oxford, pp 681–700Google Scholar
- Shuler M, Kargu F (1992) Enzymes, in bioprocess engineering basic concepts. Prentice Hall inc, New JerseyGoogle Scholar