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Biotechnology Letters

, Volume 41, Issue 2, pp 273–281 | Cite as

The marine Gram-negative bacterium Novosphingobium sp. PP1Y as a potential source of novel metabolites with antioxidant activity

  • Ganna Petruk
  • Mariana Roxo
  • Federica De Lise
  • Francesca Mensitieri
  • Eugenio Notomista
  • Michael Wink
  • Viviana IzzoEmail author
  • Daria Maria MontiEmail author
Original Research Paper
  • 88 Downloads

Abstract

Objective

The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated.

Results

The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. It showed strong protective activity against oxidative stress, in both experimental systems, by preventing ROS accumulation. In the case of the cells, pre-treatment with methanolic extract was also able to maintain unaltered intracellular GSH levels and phosphorylation levels of mitogen-activated protein kinases p38. Instead, in the case of the worms, the extract was able to modulate the expression levels of stress response genes, by activating the transcription factor skn-1.

Conclusions

From a biotechnological and economical point of view, antioxidants from microorganisms are convenient as they provide a valid alternative to chemical synthesis and respond to the ever-growing market demand for natural antioxidants.

Keywords

Antioxidants Cellular stress Sphingomonadales Novosphingobium Gram-negative bacterium Carotenoids 

Notes

Supporting information

Supplementary Fig. S1—Fractional analysis of PP1Y extract by reverse-phase HPLC.

Supplementary Fig. S2—UV–Vis spectra of peak 1 (A) and peak 2 (B). As evident from spectra, both peaks showed absorbance in visible region, with a maximum of absorbance at 369 and 447 nm for peak 1 and 369 and 442 nm for peak 2.

Author Contributions

DMM and EN conceived the study; GP conceived the study and performed research; FDL, FM and MR performed research; DMM, MW and VI analyzed data and wrote the paper; EN wrote the paper.

Compliance with ethical standards

Conflicts of Interest

The authors declare no conflict of interest.

Supplementary material

10529_2018_2636_MOESM1_ESM.pdf (146 kb)
Supplementary material 1 (PDF 146 kb)

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Copyright information

© Springer Media B.V. 2018

Authors and Affiliations

  1. 1.Department of Chemical SciencesUniversity Federico II of NaplesNaplesItaly
  2. 2.Institute of Pharmacy and Molecular BiotechnologyHeidelberg UniversityHeidelbergGermany
  3. 3.Department of BiologyUniversity Federico II of NaplesNaplesItaly
  4. 4.Department of Medicine, Surgery and DentistrySalernoItaly

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