Dexamethasone downregulates SIRT1 and IL6 and upregulates EDN1 genes in stem cells derived from gingivae via the AGE/RAGE pathway
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To evaluate the effects of dexamethasone on the aging of mesenchymal stem cells from human gingiva using next-generation sequencing.
Four mRNAs were upregulated and 12 were downregulated when the results of dexamethasone at 24 h were compared with the control at 24 h. Expressions of SIRT1 and IL6 were decreased in dexamethasone at 24 h but expression of EDN1 was increased.
Application of dexamethasone reduced the expression of SIRT1 and IL6 but enhanced the expression of EDN1 of stem cells.
KeywordsAging Dexamethasone Gingiva Messenger RNA Stem cells
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, Information and Communication Technology & Future Planning (NRF-2017R1A1A1A05001307).
Supplementary Fig. 1—Evaluation of stem cell surface marker expression (A) CD90 expression, (B) CD73 expression, (C) CD 34 expression.
Supplementary Fig. 2—Scatter plots of the expression of each green (A) Control at 24 h/control at 2 h, (B) dexamethasone at 24 h/dexamethasone at 2 h, (C) dexamethasone at 2 h/control at 2 h, (D) dexamethasone at 24 h/control at 24 h.
Supplementary Fig. 3—Gene ontology analysis of mRNA expression (A) Control at 24 h/control at 2 h, (B) dexamethasone at 24 h/dexamethasone at 2 h, (C) dexamethasone at 2 h/control at 2 h, (D) dexamethasone at 24 h/control at 24 h, (E) Venn diagram analysis (fold change 1.5, log102 normalized read counts > 4 were selected, P < 0.05).
Supplementary Table 1—Differentially expressed mRNA related to aging (fold change 1.5, log102 normalized read counts > 4 were selected, P < 0.05).
Compliance with ethical standard
Conflict of interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
The Institutional Review Board of Seoul St Mary’s Hospital (KC11SISI0348) approved of this study, and informed consent was obtained from the participants. All of the methods were performed in accordance with the relevant guidelines and regulations.
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