Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes
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To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights.
Cellulosimicrobium (family Promicromonosporaceae) and all genera of the family Cellulomonadeceaec produced both cellulosome-like multienzyme complexes and extracellular β-xylosidase activity, while the other genera of the family Promicromonosporaceae did not. Multiple sequence alignments further indicated that hypothetic protein M768_06655 might be a possible key subunit.
This is the first report that many actinobacteria species can produce cellulosome-like multienzyme complexes. The production of cellulosome-like complexes and the extracellular β-xylosidase activity against 7-xylosyltaxanes might be used to differentiate the genus Cellulosimicrobium from other genera of the family Promicromonosporaceae.
KeywordsCellulomonadeceae Cellulosome Multienzyme complexes Promicromonosporaceae β-Xylosidase 7-Xylosyltaxanes
This work was supported by the National Natural Science Foundation of China (No. 31600641) and the Fundamental Research Funds for the Central Universities (No. DUT16RC(3)016).
Supplementary Fig. 1—Multiple sequence alignment results (related to Table 2).
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