Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris
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To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production.
Monoamine oxidase, originating from Aspergillus niger and subjected to directed evolution (MAO-N D5), was cloned and expressed in Pichia pastoris CBS7435 MutS strain for the first time. Various transformants were screened at microscale level. The production of the clone expressing the most active enzyme was scaled-up to a 1.5 l fermenter and preparation of MAO-N D5 as a crude enzyme extract was optimised. The obstacles in the production of the enzyme in both expression systems, Escherichia coli and P. pastoris, are discussed and demonstrated.
There was an improvement in specific productivity, which was 83 times higher in P. pastoris, clearly proving the importance of choosing the right expression host system for the specific enzymes.
KeywordsCloning Monoamine oxidase-N D5 Pichia pastoris Upscale
The research leading to these results has received funding from the European Union Seventh Framework Programme BIONEXGEN under grant agreement no. 266025. This work was co-funded by the Slovak Research and Development Agency under contract no. DO7RP-0042-11 and by the Slovak Grant Agency for Science VEGA 2/0090/16. This work was also supported by ESF COST Chemistry Action “Systems Biocatalysis” (CM1303). Part of this work was supported acib, cofinanced by the Federal Ministry of Science, Research and Economy (BMWFW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol and ZIT—Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG.
Supplementary material—Materials and methods, Cultivation scale-up and Biotransformation.
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