High-content screening of Aspergillus niger with both increased production and high secretion rate of glucose oxidase
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To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger.
A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extracellular GOD activity was achieved in 96-well plates. There was a close negative correlation between the total GOD activity and its residual glucose of the fermentation broth. Based on this, a rapid, plate-based, qualitative analysis method of the total GOD activity was developed. Compared with the conventional analysis method using o-dianisidine, a correlation coefficient of −0.92 by statistical analysis was obtained.
Using this dual-parameter screening method, we acquired a strain with GOD activity of 3126 U l−1, which was 146% higher than the original strain. Its secretion rate of GOD was 83, 32% higher than the original strain.
KeywordsAspergillus niger Calcium carbonate Dual-parameter screening Glucose oxidase Plate-based screening Secretion rate
This work was financially supported by National Major Scientific and Technological Special Project (2012YQ15008709) and National Key Research and Development Program (2017YFB0309302).
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