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Biotechnology Letters

, Volume 39, Issue 10, pp 1485–1492 | Cite as

miR-34a attenuates glioma cells progression and chemoresistance via targeting PD-L1

  • Yi Wang
  • Li WangEmail author
Original Research Paper

Abstract

Objective

To investigate the roles of miR-34a in progression and chemoresistance of glioma cells.

Results

Quantitative real-time PCR analysis showed that miR-34a level was lower, but PD-L1 expression level was higher in glioma tissue specimens compared with normal brain tissues and their expression levels were negatively correlated. Ectopic expression of miR-34a inhibited glioma cell proliferation, promoted cell cycle arrest in G1/S phase and cell apoptosis. Additionally, miR-34a/PD-L1 axis was again confirmed and co-expression of PD-L1 with miR-34a mimics attenuated the effects of miR-34a on cell proliferation and apoptosis in glioma cells. Importantly, PD-L1 overexpression resulted in chemoresistance in glioma cells, this effect was attenuated by miR-34a overexpression.

Conclusions

miR-34a inhibits glioma cells progression and chemoresistance via targeting PD-L1.

Keywords

Drug resistance Glioma miR-34a Paclitaxel PD-L1 

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Department of NeurosurgeryCangzhou Central HospitalCangzhouChina
  2. 2.Department of OncologyThe Third Affiliated Hospital of Hebei Medical UniversityShijiazhuang CityChina

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