Biotechnology Letters

, Volume 39, Issue 4, pp 553–560 | Cite as

Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides

  • Chenghong Wang
  • Jiguo Qiu
  • Youjian Yang
  • Jinwei Zheng
  • Jian He
  • Shunpeng Li
Original Research Paper



To identify and characterize a novel aryloxyphenoxypropionate (AOPP) herbicide-hydrolyzing carboxylesterase from Aquamicrobium sp. FPB-1.


A carboxylesterase gene, fpbH, was cloned from Aquamicrobium sp. FPB-1. The gene is 798 bp long and encodes a protein of 265 amino acids. FpbH is smaller than previously reported AOPP herbicide-hydrolyzing carboxylesterases and shares only 21–35% sequence identity with them. FpbH was expressed in Escherichia coli BL21(DE3) and the product was purified by Ni–NTA affinity chromatography. The purified FpbH hydrolyzed a wide range of AOPP herbicides with catalytic efficiency in the order: haloxyfop-P-methyl > diclofop-methyl > fenoxaprop-P-ethyl > quizalofop-P-ethyl > fluazifop-P-butyl > cyhalofop-butyl. The optimal temperature and pH for FpbH activity were 37 °C and 7, respectively.


FpbH is a novel AOPP herbicide-hydrolyzing carboxylesterase; it is a good candidate for mechanistic study of AOPP herbicide-hydrolyzing carboxylesterases and for bioremediation of AOPP herbicide-contaminated environments.


Aquamicrobium sp. FPB-1 Aryloxyphenoxypropionate herbicide Biodegradation Carboxylesterase FpbH 



This work was supported by the National Natural Science Foundation of China (31270157 and 31560033) and the Project of University-Industry Collaboration of Guangdong Province-Ministry (2013B090500017).

Supplementary information

Supplementary Fig. 1—Phylogenetic tree based on the 16S rRNA gene sequences of strain FPB-1 and related species.

Supplementary Fig. 2—SDS-PAGE of His-tagged FpbH.

Supplementary Fig. 3—HPLC and tandem mass spectrometry analysis of metabolite generated during fluazifop-P-butyl degradation by the purified FpbH.

Supplementary Fig. 4—Characterization of purified recombinant FpbH.

Supplementary material

10529_2016_2276_MOESM1_ESM.docx (452 kb)
Supplementary material 1 (DOCX 451 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Chenghong Wang
    • 2
  • Jiguo Qiu
    • 1
  • Youjian Yang
    • 1
  • Jinwei Zheng
    • 3
  • Jian He
    • 1
  • Shunpeng Li
    • 1
  1. 1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life SciencesNanjing Agricultural UniversityNanjingChina
  2. 2.College of Basic Medical ScienceJiujiang UniversityJiujiangChina
  3. 3.College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingChina

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