Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp.
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With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.
The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.
Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.
KeywordsCarbazole Dibenzothiophene Polyaromatic compound Pseudomonas Rhodococcus
We are thankful to Benjamin Stark for providing pRESX. We would also like to thank the Department of Science and Technology, Government of India, for their generous financial support (Project No. SB/YS/LS-223/2013) and Jaypee Institute of Information Technology for providing research fellowship (SK).
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