Biotechnology Letters

, Volume 39, Issue 2, pp 277–281 | Cite as

Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp.

  • Samiya Khan
  • D. K. Adhikari
  • Sanjay Gupta
  • Nidhi GuptaEmail author
Original Research Paper



With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.


The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.


Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.


Carbazole Dibenzothiophene Polyaromatic compound Pseudomonas Rhodococcus 



We are thankful to Benjamin Stark for providing pRESX. We would also like to thank the Department of Science and Technology, Government of India, for their generous financial support (Project No. SB/YS/LS-223/2013) and Jaypee Institute of Information Technology for providing research fellowship (SK).


  1. Gaffney JS, Marley NA (2009) The impacts of combustion emission on air quality and climate-from coal to biofuels and beyond. Atmos Environ 43:23–36CrossRefGoogle Scholar
  2. Khan S, Adhikari DK, Gupta S, Gupta N (2015) High-level expression, purification and characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5. Biotechnol Lett 37:1945–1952CrossRefPubMedGoogle Scholar
  3. Kilbane JJ II (2006) Microbial biocatalyst developments to upgrade fossil fuels. Curr Opin Biotechnol 17:305–314CrossRefPubMedGoogle Scholar
  4. Larentis AL, Sampaio C, Carneiro C, Martins B, Alves M (2011) Evaluation of growth, carbazole biodegradation and anthranilic acid production by Pseudomonas stutzeri. Braz J Chem Eng 28:34–44CrossRefGoogle Scholar
  5. Nojiri H (2012) Structural and molecular genetic analyses of the bacterial carbazole degradation. Biosci Biotechnol Biochem 76:1–18CrossRefPubMedGoogle Scholar
  6. Ramirez-Corridores MM, Borole AP (2007) Biocatalysis in oil refining. Elsevier, AmsterdamGoogle Scholar
  7. Singh GB, Gupta S, Gupta N (2013) Carbazole degradation and biosurfactant production by newly isolated Pseudomonas sp. Strain GBS.5. Int Biodeterior Biodegrad 84:35–43CrossRefGoogle Scholar
  8. Song C, Ma X (2003) New design approaches to ultra-clean diesel fuels by deep desulfurization and deep dearomatization. Appl Catal B 41:207–238CrossRefGoogle Scholar
  9. Yu B, Ma C, Zhou W, Zhu S, Wang Y, Qu J, Li F, Xu P (2006) Simultaneous biodetoxification of s, n, and o pollutants by engineering of a carbazole-degrading gene cassette in a recombinant biocatalyst. Appl Environ Microbiol 72:7373–7376CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Samiya Khan
    • 1
  • D. K. Adhikari
    • 2
  • Sanjay Gupta
    • 1
  • Nidhi Gupta
    • 1
    Email author
  1. 1.Department of BiotechnologyJaypee Institute of Information TechnologyNoidaIndia
  2. 2.Biofuels Division & HOA Biotechnology Conversion AreaIndian Institute of PetroleumDehradunIndia

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