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Biotechnology Letters

, Volume 39, Issue 2, pp 277–281 | Cite as

Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp.

  • Samiya Khan
  • D. K. Adhikari
  • Sanjay Gupta
  • Nidhi GuptaEmail author
Original Research Paper

Abstract

Objectives

With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.

Results

The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.

Conclusions

Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.

Keywords

Carbazole Dibenzothiophene Polyaromatic compound Pseudomonas Rhodococcus 

Notes

Acknowledgements

We are thankful to Benjamin Stark for providing pRESX. We would also like to thank the Department of Science and Technology, Government of India, for their generous financial support (Project No. SB/YS/LS-223/2013) and Jaypee Institute of Information Technology for providing research fellowship (SK).

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Samiya Khan
    • 1
  • D. K. Adhikari
    • 2
  • Sanjay Gupta
    • 1
  • Nidhi Gupta
    • 1
    Email author
  1. 1.Department of BiotechnologyJaypee Institute of Information TechnologyNoidaIndia
  2. 2.Biofuels Division & HOA Biotechnology Conversion AreaIndian Institute of PetroleumDehradunIndia

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