Promigratory and proangiogenic effects of AdipoRon on bone marrow-derived mesenchymal stem cells: an in vitro study
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To investigate the effect of AdipoRon on major factors involved in survival, migration and neovascularization of rat bone marrow-derived mesenchymal stem cells.
AdipoRon promoted the MSCs viability. Real-time PCR indicated that the expression of cyclooxygenase-2 (COX-2), hypoxia-inducible factor-1 (HIF-1) C-X-C chemokine receptor type 4 (CXCR4), C–C chemokine receptor type 2 (CCR2), vascular endothelial growth factor matrix metalloproteinase-2 (MMP-2) and MMP-9 were upregulated in AdipoRon-treated MSCs compared to control groups. Prostaglandin E2 (PGE2) level, as well as migration ability of MSCs (scratch assay) was enhanced by AdipoRon preconditioning.
Preconditioning of MSCs with AdipoRon prior to transplantation could enhance cell survival, angiogenesis and migration via activating the COX-2/PGE2/HIF-1 pathway and other contributing factors.
KeywordsAdiponectin AdipoRon Cyclooxygenase-2 HIF-1 Mesenchymal stem cell Prostaglandin E2
This study was supported by a grant from Hamadan University of Medical Sciences and Iranian Council of Stem Cell Technology.
Supplementary Table 1—Primer sequences used in the quantitative Real-time PCR.
Supplementary Fig. 1—Characterization of rat MSCs.
Compliance with ethical standards
Conflict of interest
The authors declare they have no conflict of interests.
This study was approved by Ethics Committee of Hamadan University of Medical Sciences based on National Institutes of Health Principles of Laboratory Animal Care (NIH Publication No. 85–23, revised 1985).
- Huang B, Qian J, Ma J, Huang Z, Shen Y, Chen X, Sun A, Ge J, Chen H (2014) Myocardial transfection of hypoxia-inducible factor-1α and co-transplantation of mesenchymal stem cells enhance cardiac repair in rats with experimental myocardial infarction. Stem Cell Res Ther 5:22CrossRefPubMedPubMedCentralGoogle Scholar