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Biotechnology Letters

, Volume 39, Issue 1, pp 39–44 | Cite as

Promigratory and proangiogenic effects of AdipoRon on bone marrow-derived mesenchymal stem cells: an in vitro study

  • Sara Malih
  • Massoud Saidijam
  • Kamran Mansouri
  • Mona Pourjafar
  • Maryam Sadat Tafakh
  • Fahimeh Talebzadeh
  • Rezvan NajafiEmail author
Original Research Paper

Abstract

Objectives

To investigate the effect of AdipoRon on major factors involved in survival, migration and neovascularization of rat bone marrow-derived mesenchymal stem cells.

Results

AdipoRon promoted the MSCs viability. Real-time PCR indicated that the expression of cyclooxygenase-2 (COX-2), hypoxia-inducible factor-1 (HIF-1) C-X-C chemokine receptor type 4 (CXCR4), C–C chemokine receptor type 2 (CCR2), vascular endothelial growth factor matrix metalloproteinase-2 (MMP-2) and MMP-9 were upregulated in AdipoRon-treated MSCs compared to control groups. Prostaglandin E2 (PGE2) level, as well as migration ability of MSCs (scratch assay) was enhanced by AdipoRon preconditioning.

Conclusion

Preconditioning of MSCs with AdipoRon prior to transplantation could enhance cell survival, angiogenesis and migration via activating the COX-2/PGE2/HIF-1 pathway and other contributing factors.

Keywords

Adiponectin AdipoRon Cyclooxygenase-2 HIF-1 Mesenchymal stem cell Prostaglandin E2 

Notes

Acknowledgments

This study was supported by a grant from Hamadan University of Medical Sciences and Iranian Council of Stem Cell Technology.

Supporting information

Supplementary Table 1—Primer sequences used in the quantitative Real-time PCR.

Supplementary Fig. 1—Characterization of rat MSCs.

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflict of interests.

Ethical approval

This study was approved by Ethics Committee of Hamadan University of Medical Sciences based on National Institutes of Health Principles of Laboratory Animal Care (NIH Publication No. 85–23, revised 1985).

Supplementary material

10529_2016_2214_MOESM1_ESM.docx (71 kb)
Supplementary material 1 (DOCX 70 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Sara Malih
    • 1
  • Massoud Saidijam
    • 1
  • Kamran Mansouri
    • 2
  • Mona Pourjafar
    • 1
  • Maryam Sadat Tafakh
    • 1
  • Fahimeh Talebzadeh
    • 1
  • Rezvan Najafi
    • 1
    • 3
    Email author
  1. 1.Research Center for Molecular MedicineHamadan University of Medical SciencesHamadanIran
  2. 2.Medical Biology Research CenterKermanshah University of Medical SciencesKermanshahIran
  3. 3.Endometrium and Endometriosis Research CenterHamadan University of Medical SciencesHamadanIran

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