Biotechnology Letters

, Volume 38, Issue 9, pp 1603–1610

Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain

Original Research Paper

DOI: 10.1007/s10529-016-2143-y

Cite this article as:
Huang, X., Chen, M., Li, J. et al. Biotechnol Lett (2016) 38: 1603. doi:10.1007/s10529-016-2143-y

Abstract

Objectives

To develop an efficient gene-targeting platform in an excellent itaconic acid producing strain Aspergillus terreus CICC40205.

Results

The frequency of homologous recombination was improved by deleting the ku80 gene. A nutritional transformation system based on the bidirectionally selectable marker, pyrGAn, was established in the ku80-/pyrG-double mutant which is convenient for following marker rescue. The modified Cre/loxP recombination system was applied for the excision of the pyrGAn marker by directly introducing Cre recombinase into the protoplasts.

Conclusions

This gene-targeting system is an efficient platform for sequential and multiple genetic modifications in A. terreus and is conducive to study biosynthesis mechanisms and to improve the production ability of itaconic acid and other products.

Keywords

Aspergillus terreus Cre/loxP system Gene-targeting Itaconic acid Non-homologous end joining pyrG 

Supplementary material

10529_2016_2143_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1492 kb)

Funding information

Funder NameGrant NumberFunding Note
National Natural Sciences Foundation of China
  • 31400080
  • 31500042
National High Technology Research and Development Program of China
  • 2015AA021003
The Key Research Program of the Chinese Academy of Sciences
  • KSZD-EW-Z-016

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Xuenian Huang
    • 1
    • 2
  • Mei Chen
    • 1
  • Jianjun Li
    • 1
    • 3
  • Xuefeng Lu
    • 1
  1. 1.Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.National Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingChina

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