Biotechnology Letters

, Volume 38, Issue 9, pp 1443–1448 | Cite as

Enterococcus faecalis promotes osteoclast differentiation within an osteoblast/osteoclast co-culture system

  • Zuhui Deng
  • Shuai Wang
  • Boon Chin Heng
  • Changyong Yuan
  • Chengfei Zhang
Original Research Paper

Abstract

Objective

To investigate the effects of heat-killed Enterococcus faecalis ATCC 29212 and P25RC clinical strain (derived from an obturated root canal with apical periodontitis) on osteoclast differentiation within an osteoblast/osteoclast co-culture system.

Results

Heat-killed E. faecalis significantly increased the proportion of multinucleated osteoclastic cells (MNCs) within the co-culture system. The IL-6 level was significantly increased upon exposure to heat-killed E. faecalis. Gene expression levels of NFATc1 and cathepsin K were significantly up-regulated compared to the untreated control. EphrinB2 and EphB4 expressions at both the mRNA and protein levels were also significantly upregulated compared to the untreated control.

Conclusions

Heat-killed E. faecalis can induce osteoclast differentiation within the osteoblast/osteoclast co-culture system in vitro, possibly through ephrinB2-EphB4 bidirectional signaling.

Keywords

Enterococcus faecalis EphrinB2 EphB4 Multinucleated osteoclastic cells Osteoblasts Osteoclasts Osteoclast differentiation 

Notes

Acknowledgments

This work was supported by RFCID/HHSRF HMRF Grant No. 12110772 and Shenzhen Key Laboratory of ENT (No. ZDSYS 201506050935272).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Zuhui Deng
    • 1
    • 2
  • Shuai Wang
    • 1
  • Boon Chin Heng
    • 1
  • Changyong Yuan
    • 1
  • Chengfei Zhang
    • 1
  1. 1.Endodontics, Comprehensive Dental Care, Faculty of DentistryThe University of Hong KongHong Kong SARChina
  2. 2.Shenzhen ENT InstituteShenzhen Longgang ENT HospitalShenzhenChina

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