Biotechnology Letters

, Volume 38, Issue 8, pp 1359–1365 | Cite as

An engineered non-oxidative glycolysis pathway for acetone production in Escherichia coli

  • Xiaoyan Yang
  • Qianqian Yuan
  • Yangyang Zheng
  • Hongwu MaEmail author
  • Tao Chen
  • Xueming Zhao
Original Research Paper



To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli.


Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical acetone yield from 1 to 1.5 mol acetone/mol glucose. By inserting the fxpk gene encoding phosphoketolase from Bifidobacterium adolescentis into the genome, we constructed a NOG pathway in E.coli. The resulting strain produced 47 mM acetone from glucose under aerobic conditions in shake-flasks. The yield of acetone was improved from 0.38 to 0.47 mol acetone/mol glucose which is a significant over the parent strain.


Guided by computational analysis of metabolic networks, we introduced a NOG pathway into E. coli and increased the yield of acetone, which demonstrates the importance of modeling analysis for the novel metabolic engineering strategies.


Acetone Escherichia coli Non-oxidative glycolysis Phosphoketolase 



This work was supported by the National Key Basic Research Program of China (973 Program) (Nos. 2012CB725203, 2015CB755704) and the Key Project in the Tianjin Science & Technology Pillar Program (No. 14ZCZDSY00060).

Supporting Information

Supplementary Table 1—Strains and plasmids used in this study.

Supplementary Table 2—Primer sequence used in this study.

Supplementary material

10529_2016_2115_MOESM1_ESM.doc (61 kb)
Supplementary material 1 (DOC 61 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Xiaoyan Yang
    • 1
    • 2
  • Qianqian Yuan
    • 1
    • 2
  • Yangyang Zheng
    • 2
  • Hongwu Ma
    • 2
    Email author
  • Tao Chen
    • 1
  • Xueming Zhao
    • 1
  1. 1.School of Chemical Engineering and TechnologyTianjin UniversityTianjinPeople’s Republic of China
  2. 2.Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjinPeople’s Republic of China

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