Biotechnology Letters

, Volume 38, Issue 7, pp 1181–1186 | Cite as

Enhanced succinic acid production in Corynebacterium glutamicum with increasing the available NADH supply and glucose consumption rate by decreasing H+-ATPase activity

  • Hongtao Xu
  • Zhihui Zhou
  • Chen Wang
  • Zhongjun Chen
  • Heng CaiEmail author
Original Research Paper



To enhance succinic acid production in Corynebacterium glutamicum by increasing the supply of NADH and the rate of glucose consumption by decreasing H+-ATPase activity.


A mutant of C. glutamicum NC-3-1 with decreased H+-ATPase activity was constructed. This increased the rate of glycolysis and the supply of NADH. Fermentation of C. glutamicum NC-3-1 gave 39 % higher succinic acid production (113 and 81 g/l), a 29 % higher succinic acid yield (0.94 and 0.73 g succinic acid/g glucose) and decreased by-products formation compared to that of C. glutamicum NC-3 in 5 l bioreactor.


The point mutation in C. glutamicum NC-3-1 increased the rate of glycolysis and resulted in higher succinic acid production, higher succinic acid yield and significantly decreased formation of by-products.


Corynebacterium glutamicum Glucose consumption rate H+-ATPase defect NADH supply Succinic acid 



This work was supported by the 973 Program of China (grant no. 2011CB707405).

Supporting information

Anaerobic succinic acid production in a seated bottle.

Anaerobic succinic acid production in a 5 l bioreactor.

Supplementary Table 1—Sequences of oligonucleotide primers.

Supplementary material

10529_2016_2093_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 34 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Hongtao Xu
    • 1
  • Zhihui Zhou
    • 1
  • Chen Wang
    • 1
  • Zhongjun Chen
    • 2
  • Heng Cai
    • 1
    Email author
  1. 1.College of Biotechnology and Pharmaceutical EngineeringNanjing University of TechnologyNanjingChina
  2. 2.College of Food Science and EngineeringInner Mongolia Agricultural UniversityHohhotChina

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