Biotechnology Letters

, Volume 38, Issue 6, pp 975–982 | Cite as

Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production

  • Chelladurai Rathnasingh
  • Jong Myoung Park
  • Duk-ki Kim
  • Hyohak Song
  • Yong Keun Chang
Original Research Paper



To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated.


Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta-ackA strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation.


K. pneumoniae knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity.


Acetate kinase Alcohol dehydrogenase 2,3-Butanediol In silico investigation Klebsiella pneumoniae Lactate dehydrogenase Phosphotransacetylase 



This work was supported by the Industrial Strategic Technology Development Program (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) and the Advanced Biomass R&D Center of Global Frontier Project (No. ABC-2010-0029728) funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea).


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Chelladurai Rathnasingh
    • 1
  • Jong Myoung Park
    • 1
  • Duk-ki Kim
    • 1
    • 2
  • Hyohak Song
    • 1
  • Yong Keun Chang
    • 2
  1. 1.Research and Development CenterGS Caltex CorporationDaejeonRepublic of Korea
  2. 2.BioSystem Engineering Laboratory, Department of Chemical and Biomolecular EngineeringKAISTDaejeonRepublic of Korea

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