Biotechnology Letters

, Volume 38, Issue 5, pp 793–799 | Cite as

Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice

  • Stella Siaw Xiu Joan
  • Jee Pui-Fong
  • Adelene Ai-Lian Song
  • Li-Yen Chang
  • Khatijah Yusoff
  • Sazaly AbuBakar
  • Raha Abdul Rahim
Original Research Paper

Abstract

Objective

An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.

Results

Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.

Conclusion

Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.

Keywords

Influenza virus H1N1 Haemagglutinin Lactococcus lactis Oral vaccine Surface display Vaccine 

Supplementary material

10529_2016_2034_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Stella Siaw Xiu Joan
    • 1
  • Jee Pui-Fong
    • 4
    • 5
  • Adelene Ai-Lian Song
    • 1
  • Li-Yen Chang
    • 4
    • 5
  • Khatijah Yusoff
    • 2
    • 3
  • Sazaly AbuBakar
    • 4
    • 5
  • Raha Abdul Rahim
    • 1
    • 3
  1. 1.Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular SciencesUniversity Putra Malaysia (UPM)SerdangMalaysia
  2. 2.Department of Microbiology, Faculty of Biotechnology and Biomolecular SciencesUniversity Putra MalaysiaSerdangMalaysia
  3. 3.Institute of BioscienceUniversity Putra MalaysiaSerdangMalaysia
  4. 4.Department of Medical Microbiology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  5. 5.Tropical Infectious Diseases Research and Education Centre (TIDREC)University of MalayaKuala LumpurMalaysia

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