Biotechnology Letters

, Volume 38, Issue 3, pp 503–508 | Cite as

Metabolic engineering of Saccharomyces cerevisiae for linalool production

  • Pegah Amiri
  • Azar Shahpiri
  • Mohammad Ali Asadollahi
  • Fariborz Momenbeik
  • Siavash Partow
Original Research Paper

Abstract

Objectives

To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool.

Results

Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its native promoter with the repressible MET3 promoter in the presence of methionine resulted in accumulation of 78 µg linalool l−1 in the culture medium. This was more than twice that produced by the control strain. The highest linalool titer was obtained by combined repression of ERG9 and overexpression of tHMG1. The yeast strain harboring both modifications produced 95 μg linalool l−1.

Conclusions

Although overexpression of tHMG1 and downregulation of ERG9 enhanced linalool titers threefold in the engineered yeast strain, alleviating linalool toxicity is necessary for further improvement of linalool biosynthesis in yeast.

Keywords

Lavandula angustifolia Linalool Linalool synthase Metabolic engineering Monoterpene Saccharomyces cerevisiae 

Supplementary material

10529_2015_2000_MOESM1_ESM.docx (11 kb)
Supplementary material 1 (DOCX 11 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Pegah Amiri
    • 1
  • Azar Shahpiri
    • 1
  • Mohammad Ali Asadollahi
    • 2
  • Fariborz Momenbeik
    • 3
  • Siavash Partow
    • 4
  1. 1.Department of Agricultural Biotechnology, College of AgricultureIsfahan University of TechnologyIsfahanIran
  2. 2.Department of Biotechnology, Faculty of Advanced Sciences and TechnologiesUniversity of IsfahanIsfahanIran
  3. 3.Department of Analytical Chemistry, Faculty of ChemistryUniversity of IsfahanIsfahanIran
  4. 4.Department of Chemical Engineering & Applied ChemistryUniversity of TorontoTorontoCanada

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