Biotechnology Letters

, Volume 38, Issue 3, pp 477–481

Selective oxidation of UDP-glucose to UDP-glucuronic acid using permeabilized Schizosaccharomyces pombe expressing human UDP-glucose 6-dehydrogenase

Original Research Paper

Abstract

Objectives

To use permeabilized cells of the fission yeast, Schizosaccharomyces pombe, that expresses human UDP-glucose 6-dehydrogenase (UGDH, EC 1.1.1.22), for the production of UDP-glucuronic acid from UDP-glucose.

Results

In cell extracts no activity was detected. Therefore, cells were permeabilized with 0.3 % (v/v) Triton X-100. After washing away all low molecular weight metabolites, the permeabilized cells were directly used as whole cell biocatalyst. Substrates were 5 mM UDP-glucose and 10 mM NAD+. Divalent cations were not added to the reaction medium as they promoted UDP-glucose hydrolysis. With this reaction system 5 mM UDP-glucose were converted into 5 mM UDP-glucuronic acid within 3 h.

Conclusions

Recombinant permeabilized cells of S. pombe can be used to synthesize UDP-glucuronic acid with 100 % yield and selectivity.

Keywords

Biocatalysis Permeabilized cells Schizosaccharomyces pombe Selective oxidation UDP-glucose-6-dehydrogenase UDP-glucuronate 

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Christian Weyler
    • 1
  • Matthias Bureik
    • 2
  • Elmar Heinzle
    • 1
  1. 1.Biochemical Engineering InstituteSaarland UniversitySaarbrückenGermany
  2. 2.School of Pharmaceutical Science and Technology (SPST)Tianjin UniversityTianjinPeople’s Republic of China

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