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Biotechnology Letters

, Volume 37, Issue 12, pp 2435–2444 | Cite as

Characterization of the phenolic fraction from Argentine wine and its effect on viability and polysaccharide production of Pediococcus pentosaceus

  • María Gilda Stivala
  • Margarita Beatriz Villecco
  • Martín Fanzone
  • Viviana Jofré
  • Pedro Aredes-FernándezEmail author
Original Research Paper

Abstract

Objectives

To qualitatively and quantitatively characterize a low molecular weight phenolic fraction (LMF) of Malbec wine from Cafayate, Argentina, and evaluate its effect on viability and exopolysaccharide production of Pediococcus pentosaceus 12p, a wine spoilage bacterium.

Results

The phenolic compounds detected were, in general, comparable to data previously reported but hydroxycinnamic acids were detected at higher concentrations than determined in other studies. Addition of LMF at identical concentrations present in wine or a four times concentrated LMF mixture to a synthetic wine-like medium produced a diminution in bacterial viability and exopolysaccharide production in the supernatant culture. Transmission electron microscopy revealed damage of bacterial cell integrity after 96 h of incubation only in the presence of four times concentrated LMF.

Conclusion

This is the first time a low molecular weight phenolic fraction has been characterized in Cafayate wine and it has demonstrated a marked antimicrobial effect on an exopolysaccharide-producing wine spoilage bacterium.

Keywords

Antimicrobial activity Exopolysaccharide Low molecular weight phenolic fraction Pediococcus pentosaceus Wine 

Notes

Acknowledgments

The authors wish to thank the Agencia Nacional de Promoción Científica y Técnica (ANPCYT) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Facultad de Bioquímica, Química y FarmaciaUniversidad Nacional de TucumánSan Miguel de TucumánArgentina
  2. 2.Laboratorio de Aromas y Sustancias Naturales, Estación Experimental Agropecuaria MendozaInstituto Nacional de Tecnología Agropecuaria (INTA)Luján de CuyoArgentina

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