Isolation and characterization of an interactive culture of two Paenibacillus species with moderately thermophilic desulfurization ability
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To isolate and characterize novel thermophilic bacteria capable of biodesulfurization of petroleum.
A culture containing two Paenibacillus spp. (denoted “32O-W” and “32O-Y”) was isolated by repeated passage of a soil sample at up to 55 °C in medium containing dibenzothiophene (DBT) as sulfur source. Only 32O-Y metabolized DBT, apparently via the 4S pathway; maximum activity occurred from 40 to 45 °C, with some activity up to at least 50 °C. 32O-W enhanced DBT metabolism by 32O-Y (by 22–74 % at 40–50 °C). With sulfate as sulfur source, 32O-Y and 32O-W grew well up to 58 and 63 °C, respectively. Selection of a mixed culture of 32O-Y and 32O-W at 54 °C increased DBT metabolism 36–42 % from 40 to 45 °C. Genome sequencing identified desulfurization gene homologs in the strains consistent with their desulfurization properties.
The 32O-Y/32O-W culture may be a useful starting point for development of an improved thermophilic petroleum biodesulfurization process.
KeywordsBiodesulfurization Dibenzothiophene Directed evolution Interaction Paenibacillus Thermophiles
This work was supported by a Grant (No. 6600019855) from the Saudi Arabian Oil Company (Saudi Aramco).
Supplementary Fig. 1—Data for the identification of strain 32-Y as Paenibacillus naphthalenovorans and strain 320-W as Paenibacillus sp.
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