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Biotechnology Letters

, Volume 37, Issue 11, pp 2201–2211 | Cite as

Isolation and characterization of an interactive culture of two Paenibacillus species with moderately thermophilic desulfurization ability

  • Jia Wang
  • Batzaya Davaadelger
  • Joelle K. Salazar
  • Robert R. ButlerIII
  • Jean-François Pombert
  • John J. Kilbane
  • Benjamin C. StarkEmail author
Original Research Paper

Abstract

Objective

To isolate and characterize novel thermophilic bacteria capable of biodesulfurization of petroleum.

Results

A culture containing two Paenibacillus spp. (denoted “32O-W” and “32O-Y”) was isolated by repeated passage of a soil sample at up to 55 °C in medium containing dibenzothiophene (DBT) as sulfur source. Only 32O-Y metabolized DBT, apparently via the 4S pathway; maximum activity occurred from 40 to 45 °C, with some activity up to at least 50 °C. 32O-W enhanced DBT metabolism by 32O-Y (by 22–74 % at 40–50 °C). With sulfate as sulfur source, 32O-Y and 32O-W grew well up to 58 and 63 °C, respectively. Selection of a mixed culture of 32O-Y and 32O-W at 54 °C increased DBT metabolism 36–42 % from 40 to 45 °C. Genome sequencing identified desulfurization gene homologs in the strains consistent with their desulfurization properties.

Conclusion

The 32O-Y/32O-W culture may be a useful starting point for development of an improved thermophilic petroleum biodesulfurization process.

Keywords

Biodesulfurization Dibenzothiophene Directed evolution Interaction Paenibacillus Thermophiles 

Notes

Acknowledgments

This work was supported by a Grant (No. 6600019855) from the Saudi Arabian Oil Company (Saudi Aramco).

Supporting information

Supplementary Fig. 1—Data for the identification of strain 32-Y as Paenibacillus naphthalenovorans and strain 320-W as Paenibacillus sp.

Supplementary material

10529_2015_1918_MOESM1_ESM.doc (38 kb)
Supplementary material 1 (DOC 38 kb)
10529_2015_1918_MOESM2_ESM.eps (1.4 mb)
Supplementary material 2 (EPS 1412 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Jia Wang
    • 1
  • Batzaya Davaadelger
    • 1
  • Joelle K. Salazar
    • 1
  • Robert R. ButlerIII
    • 1
  • Jean-François Pombert
    • 1
  • John J. Kilbane
    • 1
  • Benjamin C. Stark
    • 1
    Email author
  1. 1.Department of BiologyIllinois Institute of TechnologyChicagoUSA

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