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Biotechnology Letters

, Volume 37, Issue 10, pp 2091–2096 | Cite as

Enhancement of ginsenoside Rg1 in Panax ginseng hairy root by overexpressing the α-l-rhamnosidase gene from Bifidobacterium breve

  • Ru Zhang
  • Bian-Ling ZhangEmail author
  • Gu-Cai Li
  • Tao Xie
  • Teng Hu
  • Zhi-Yong Luo
Original Research Paper

Abstract

Objectives

To improve the production of ginsenoside Rg1 in Panax ginseng.

Results

The α-l-rhamnosidase gene from Bifidobacterium breve (BbRha) was overexpressed into hairy root culture system using Agrobacterium rhizogenes A4. Ginsenoside Rg1 in hairy roots was obtained following transformation via overexpressed gene representing 2.2-fold higher than those of control lines. Several overexpression transgenic hairy root lines were obtained exhibiting markedly increased levels of the corresponding α-l-rhamnosidase enzymatic activity relative to control. Ginsenoside Rg1 levels in the transgenic lines were higher (2.2-fold) than those of control after following 30 days culturing, while ginsenoside Re contents in tested transgenic lines were found to be lower. The transgenic hairy roots harboring α-l-rhamnosidase gene improved the accumulation of ginsenoside Rg1 up to 3.6 mg g−1 dry weight.

Conclusion

BbRha gene selectively enhances the production of ginsenoside Rg1 in P. ginseng hairy roots.

Keywords

Bifidobacterium breve Ginsenoside Rg1 Hairy roots Panax ginseng α-l-Rhamnosidase Transgenic hairy roots 

Notes

Acknowledgments

The study was supported by the Natural Science Foundation of Hunan Province (11JJ6009), the Scientific Research Fund of Hunan Provincial Education Department (11C0329) and the National Training Programs of Innovation and Entrepreneurship for Undergraduates (201411342004). The critical reading of the manuscript by Dr. Mohammed Nuruzzaman, Molecular Biology Research Center, School of Life Sciences, Central South University is greatly appreciated.

Supporting information

Supplementary Table 1: PCR amplification primers of BbRha, Rol, and β-actin genes.

Supplementary material

10529_2015_1889_MOESM1_ESM.doc (41 kb)
Supplementary material 1 (DOC 41 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Ru Zhang
    • 1
  • Bian-Ling Zhang
    • 1
    Email author
  • Gu-Cai Li
    • 1
  • Tao Xie
    • 1
  • Teng Hu
    • 1
  • Zhi-Yong Luo
    • 2
  1. 1.College of Chemistry and Chemical EngineeringHunan Institute of EngineeringXiangtanChina
  2. 2.Molecular Biology Research Center, School of Life SciencesCentral South UniversityChangshaChina

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