Biotechnology Letters

, Volume 37, Issue 9, pp 1765–1771 | Cite as

Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers

Original Research Paper

Abstract

Objective

To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production.

Results

P10UTR (the 3′-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65 %. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/106 cells) compared with standard baculovirus vector.

Conclusion

The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.

Keywords

Baculovirus Porcine circovirus type 2 Sf9 cell Translational enhancers Virus-like particles 

Supplementary material

10529_2015_1856_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.College of Veterinary MedicineNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.China-UK, Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect BiologyNanyang Normal UniversityNanyangPeople’s Republic of China

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