Biotechnology Letters

, Volume 37, Issue 9, pp 1765–1771 | Cite as

Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers

  • Yangkun Liu
  • Yuanyuan Zhang
  • Lunguang Yao
  • Huafang Hao
  • Xiangjing Fu
  • Zengqi Yang
  • Enqi Du
Original Research Paper



To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production.


P10UTR (the 3′-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65 %. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/106 cells) compared with standard baculovirus vector.


The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.


Baculovirus Porcine circovirus type 2 Sf9 cell Translational enhancers Virus-like particles 



This work was supported by National Natural Science Foundation of China (Grant No. 31101803/C1803) and Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Grant No. SKLVBF201405).

Supporting information

Supplementary Table 1—Primers used in this study.

Supplementary material

10529_2015_1856_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.College of Veterinary MedicineNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.China-UK, Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect BiologyNanyang Normal UniversityNanyangPeople’s Republic of China

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