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Biotechnology Letters

, Volume 37, Issue 8, pp 1565–1571 | Cite as

Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer

  • Gong Cheng
  • Changzhen Fu
  • Hong Wang
  • C. Adoligbe
  • Shengjuan Wei
  • Shijun Li
  • Bijie Jiang
  • Hongbao Wang
  • Linsen ZanEmail author
Original Research Paper

Abstract

Objectives

Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial to human health. However, the n-3 PUFAs contents of the livestock meat that we consume daily are relatively low. Utilization of transgenic technology to increase n-3 PUFAs contents in livestock may solve this problem.

Results

The omega-3 fatty acid desaturase (FAD3), encoded by fat1 gene derived from Caenorhabditis elegans (C. elegans), converts omega-6 polyunsaturated fatty acids (n-6 PUFAs) to n-3 PUFAs. In the study, a plasmid containing the codon-optimized C. elegans fat1 gene (mfat1) was constructed and used to produce transgenic beef cattle by somatic cell nuclear transfer. Fourteen transgenic calves were obtained, and the ratio of n-6 to n-3 PUFAs in the transgenic calves decreased from 5.33: 1 to 0.95: 1 compared with negative controls.

Conclusions

Our results demonstrated that the codon-optimized C. elegans mfat1 gene can be functionally expressed in the beef cattle and converts n-6 PUFAs to n-3 PUFAs.

Keywords

Fatty acid desaturase Fat1 gene n-3 Polyunsaturated fatty acids Somatic cell nuclear transfer Transgenic cattle 

Notes

Acknowledgments

This work was supported by the GMO New Varieties Major Project (2013ZX08007-002, 2014ZX08007-002), the National Natural Science Foundation (31301937) and the Chinese National High-Tech Research and Development Program (2013AA102505).

Supporting information

Supplementary Table 1—Codon optimization of fat1 gene.

Supplementary material

10529_2015_1827_MOESM1_ESM.doc (37 kb)
Supplementary material 1 (DOC 37 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Gong Cheng
    • 1
    • 2
  • Changzhen Fu
    • 1
  • Hong Wang
    • 1
  • C. Adoligbe
    • 1
  • Shengjuan Wei
    • 1
  • Shijun Li
    • 1
  • Bijie Jiang
    • 1
  • Hongbao Wang
    • 1
    • 2
  • Linsen Zan
    • 1
    • 2
    Email author
  1. 1.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingChina
  2. 2.National Beef Cattle Improvement CentreYanglingChina

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