Recent developments in biocatalysis beyond the laboratory
Recent developments in biocatalysis, where implementation beyond the laboratory has been demonstrated, are explored: the use of transglutaminases to modify foods, reduce allergenicity and produce advanced materials, lipases for biodiesel production, and transaminases for biochemical production. The availability and application of enzymes at pilot and larger scale opens up possibilities for further improvements of biocatalyst-based processes and the development of new processes. Enzyme production, stability, activity, re-use, and product retrieval are common challenges for biocatalytic processes. We explore recent advances in biocatalysis within the process chain, such as protein engineering, enzyme expression, and biocatalyst immobilization, in the context of these challenges.
KeywordsBiocatalysis Enzyme expression Immobilization Lipases Protein engineering Transaminases Transglutaminase
Jasmina Nikodinovic-Runic is partially funded by the Ministry of Education, Science and Technological Development of Serbia Project 173048. Tanja Narancic is funded by the EC FP7 project SYNPOL (311815); Reeta Davis is funded by Science Foundation Ireland AMBER centre (12/RC/2278). Authors acknowledge the Systems Biocatalysis COST Action CM1303.
- Brena BM, Batista-Viera F (2006) Immobilization of enzymes. In: Guisan JM (ed) Methods in biotechnology: immobilization of enzymes and cells, vol 22, 2nd edn. Humana Press Inc, TotowaGoogle Scholar
- Frodsham L, Golden M, Hard S, Kenworthy MN, Klauber DJ, Leslie K, Macleod C, Meadows RE, Mulholland KR, Reilly J, Squire C, Tomasi S, Watt D, Wells AS (2013) Use of omega-transaminase enzyme chemistry in the synthesis of a JAK2 kinase inhibitor. Org Process Res Dev 17:1123–1130CrossRefGoogle Scholar
- Hervé G, Agneta F, Yves D (2011) Biofuels and World Agricultural Markets: outlook for 2020 and 2050. In: Bernardes MADS (ed) Economic effects of biofuel production. doi: 10.5772/20581 InTech, RijekaGoogle Scholar
- Markets and Markets (2013) Food enzymes market by types (carbohydrase, protease, lipase), applications (beverages, dairy, bakery), sources (microorganisms, plants, animals), and geography—Global trends and forecasts to, 2018, vol FB 1264. Markets and Markets, DallasGoogle Scholar
- Meadows RE, Mulholland KR, Schurmann M, Golden M, Kierkels H, Meulenbroeks E, Mink D, May O, Squire C, Straatman H, Wells AS (2013) Efficient synthesis of (S)-1-(5-Fluoropyrimidin-2-yl)ethylamine using an omega-transaminase biocatalyst in a two-phase system. Org Process Res Dev 17:1117–1122CrossRefGoogle Scholar
- Midelfort KS, Kumar R, Han S, Karmilowicz MJ, McConnell K, Gehlhaar DK, Mistry A, Chang JS, Anderson M, Villalobos A, Minshull J, Govindarajan S, Wong JW (2013) Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin. Protein Eng Des Sel 26:25–33CrossRefPubMedGoogle Scholar
- Olivier CE, Lima RP, Pinto DG, Santos RA, Silva GK, Lorena SL, Villas-Boas MB, Netto FM, Zollner Rde L (2012) In search of a tolerance-induction strategy for cow’s milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics 67:1171–1179CrossRefPubMedCentralPubMedGoogle Scholar
- Ramos OS, Malcata FX (2011) Food-grade enzymes. In: Moo-Young M, Butler M, Webb BC et al (eds) Comprehensive Biotechnology. Academic Press, BurlingtonGoogle Scholar
- Smerdel B, Pollak L, Novotni D, Cukelj N, Benkovic M, Lusic D, Curic D (2012) Improvement of gluten-free bread quality using transglutaminase, various extruded flours and protein isolates. J Food Nut Res 51:242–253Google Scholar