Biotechnology Letters

, Volume 37, Issue 5, pp 943–954 | Cite as

Recent developments in biocatalysis beyond the laboratory

  • Tanja Narancic
  • Reeta Davis
  • Jasmina Nikodinovic-Runic
  • Kevin E. O’ Connor
Review

Abstract

Recent developments in biocatalysis, where implementation beyond the laboratory has been demonstrated, are explored: the use of transglutaminases to modify foods, reduce allergenicity and produce advanced materials, lipases for biodiesel production, and transaminases for biochemical production. The availability and application of enzymes at pilot and larger scale opens up possibilities for further improvements of biocatalyst-based processes and the development of new processes. Enzyme production, stability, activity, re-use, and product retrieval are common challenges for biocatalytic processes. We explore recent advances in biocatalysis within the process chain, such as protein engineering, enzyme expression, and biocatalyst immobilization, in the context of these challenges.

Keywords

Biocatalysis Enzyme expression Immobilization Lipases Protein engineering Transaminases Transglutaminase 

References

  1. Adachi D, Koh F, Hama S, Ogino C, Kondo A (2013) A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: characterization and application to enzymatic biodiesel production. Enzym Microb Technol 52:331–335CrossRefGoogle Scholar
  2. Brena BM, Batista-Viera F (2006) Immobilization of enzymes. In: Guisan JM (ed) Methods in biotechnology: immobilization of enzymes and cells, vol 22, 2nd edn. Humana Press Inc, TotowaGoogle Scholar
  3. Buettner K, Hertel TC, Pietzsch M (2012) Increased thermostability of microbial transglutaminase by combination of several hot spots evolved by random and saturation mutagenesis. Amino Acids 42:987–996CrossRefPubMedGoogle Scholar
  4. Chattopadhyay S, Sen R (2013) Development of a novel integrated continuous reactor system for biocatalytic production of biodiesel. Bioresour Technol 147:395–400CrossRefPubMedGoogle Scholar
  5. Christopher LP, Kumar H, Zambare VP (2014) Enzymatic biodiesel: challenges and opportunities. Appl Energy 119:497–520CrossRefGoogle Scholar
  6. Clare DA, Daubert CR (2011) Expanded functionality of modified whey protein dispersions after transglutaminase catalysis. J Food Sci 76:C576–C584CrossRefPubMedGoogle Scholar
  7. Dach R, Song JHJ, Roschangar F, Samstag W, Senanayake CH (2012) The eight criteria defining a good chemical manufacturing process. Org Process Res Dev 16:1697–1706CrossRefGoogle Scholar
  8. de Goes-Favoni S, Bueno FR (2014) Microbial transglutaminase: general characteristics and performance in food processing technology. Food Biotechnol 28:1–24CrossRefGoogle Scholar
  9. De Jong GAH, Koppelman SJ (2002) Transglutaminase catalyzed reactions: impact on food applications. J Food Sci 67:2798–2806CrossRefGoogle Scholar
  10. Di Pierro P, Rossi Marquez G, Mariniello L, Sorrentino A, Villalonga R, Porta R (2013) Effect of transglutaminase on the mechanical and barrier properties of whey protein/pectin films prepared at complexation pH. J Agr Food Chem 61:4593–4598CrossRefGoogle Scholar
  11. Dunn PJ (2012) The importance of green chemistry in process research and development. Chem Soc Rev 41(4):1452–1461CrossRefPubMedGoogle Scholar
  12. Erickson DP, Campanella OH, Hamaker BR (2012) Functionalizing maize zein in viscoelastic dough systems through fibrous, beta-sheet-rich protein networks: an alternative, physicochemical approach to gluten-free breadmaking. Trends Food Sci Technol 24:74–81CrossRefGoogle Scholar
  13. Frodsham L, Golden M, Hard S, Kenworthy MN, Klauber DJ, Leslie K, Macleod C, Meadows RE, Mulholland KR, Reilly J, Squire C, Tomasi S, Watt D, Wells AS (2013) Use of omega-transaminase enzyme chemistry in the synthesis of a JAK2 kinase inhibitor. Org Process Res Dev 17:1123–1130CrossRefGoogle Scholar
  14. Gerrard JA, Fayle SE, Brown PA, Sutton KH, Simmons L, Rasiah I (2001) Effects of microbial transglutaminase on the wheat proteins of bread and croissant dough. J Food Sci 66:782–786CrossRefGoogle Scholar
  15. Giosafatto CVL, Rigby NM, Wellner N, Ridout M, Husband F, Mackie AR (2012) Microbial transglutaminase-mediated modification of ovalbumin. Food Hydrocoll 26:261–267CrossRefGoogle Scholar
  16. Girardin M, Ouellet SG, Gauvreau D, Moore JC, Hughes G, Devine PN, O’shea PD, Campeau LC (2013) Convergent kilogram-scale synthesis of dual orexin receptor antagonist. Org Process Res Dev 17:61–68CrossRefGoogle Scholar
  17. Gog A, Roman M, Tos M, Paizs C, Irimie FD (2012) Biodiesel production using enzymatic transesterification—current state and perspectives. Renew Energy 39:10–16CrossRefGoogle Scholar
  18. Green AP, Turner NJ, O’Reilly E (2014) Chiral amine synthesis using omega-transaminases: an amine donor that displaces equilibria and enables high-throughput screening. Angew Chem Int Ed Engl 53:10714–10717CrossRefPubMedGoogle Scholar
  19. Hama S, Kondo A (2013) Enzymatic biodiesel production: an overview of potential feedstocks and process development. Bioresour Technol 135:386–395CrossRefPubMedGoogle Scholar
  20. Han LH, Cheng YQ, Qiu S, Tatsumi E, Shen Q, Lu ZH, Li LT (2013) The effects of vital wheat gluten and transglutaminase on the thermomechanical and dynamic rheological properties of buckwheat dough. Food Bioprocess Technol 6:561–569CrossRefGoogle Scholar
  21. Heredia-Sandoval NG, Islas-Rubio AR, Cabrera-Chavez F, De la Barca AMC (2014) Transamidation of gluten proteins during the bread-making process of wheat flour to produce breads with less immunoreactive gluten. Food Funct 5:1813–1818CrossRefPubMedGoogle Scholar
  22. Hervé G, Agneta F, Yves D (2011) Biofuels and World Agricultural Markets: outlook for 2020 and 2050. In: Bernardes MADS (ed) Economic effects of biofuel production. doi: 10.5772/20581 InTech, RijekaGoogle Scholar
  23. Huang J, Xia J, Yang Z, Guan F, Cui D, Guan G, Jiang W, Li Y (2014) Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel. Biotechnol Biofuels 7:111CrossRefPubMedCentralPubMedGoogle Scholar
  24. Huisman GW, Collier SJ (2013) On the development of new biocatalytic processes for practical pharmaceutical synthesis. Curr Opin Chem Biol 17:284–292CrossRefPubMedGoogle Scholar
  25. Hwang BY, Kim BG (2004) High-throughput screening method for the identification of active and enantioselective omega-transaminases. Enzyme Micro Technol 34:429–436CrossRefGoogle Scholar
  26. Hwang YT, Qi F, Yuan C, Zhao X, Ramkrishna D, Liu D, Varma A (2014) Lipase catalyzed process for biodiesel production: protein engineering and lipase production. Biotechnol Bioeng 111:639–653CrossRefPubMedGoogle Scholar
  27. Illanes A, Cauerhff A, Wilson L, Castro GR (2012) Recent trends in biocatalysis engineering. Bioresour Technol 115:48–57CrossRefPubMedGoogle Scholar
  28. James J, Simpson BK (1996) Application of enzymes in food processing. Crit Rev Food Sci Nutr 36:437–463CrossRefPubMedGoogle Scholar
  29. Jegannathan KR, Nielsen PH (2013) Environmental assessment of enzyme use in industrial production: a literature review. J Cleaner Prod 42:228–240CrossRefGoogle Scholar
  30. Jin Z, Han SY, Zhang L, Zheng SP, Wang Y, Lin Y (2013) Combined utilization of lipase displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co solvent media. Bioresour Technol 130:102–109CrossRefPubMedGoogle Scholar
  31. Kanaji T, Ozaki H, Takano T, Ide H, Motoki M, Shimonishi Y (1993) Primary structure of microbial transglutaminase from Streptoverticillium sp. strain S-8112. J Biol Chem 268:11565–11572PubMedGoogle Scholar
  32. Kieliszek M, Misiewicz A (2014) Microbial transglutaminase and its application in the food industry: a review. Folia Microbiol 59:241–250CrossRefGoogle Scholar
  33. Kohls H, Steffen-Munsberg F, Hohne M (2014) Recent achievements in developing the biocatalytic toolbox for chiral amine synthesis. Curr Opin Chem Biol 19:180–192CrossRefPubMedGoogle Scholar
  34. Korman TP, Sahachartsiri B, Charbonneau DM, Huang GL, Beauregard M, Bowie JU (2013) Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution. Biotechnol Biofuels 6:70CrossRefPubMedCentralPubMedGoogle Scholar
  35. Kroutil W, Fischereder EM, Fuchs CS, Lechner H, Mutti FG, Pressnitz D, Rajagopalan A, Sattler JH, Simon RC, Siirola E (2013) Asymmetric preparation of prim-, sec-, and tert-amines employing selected biocatalysts. Org Process Res Dev 17(5):751–759CrossRefPubMedCentralPubMedGoogle Scholar
  36. Kuraishi C, Yamazaki K, Susa Y (2001) Transglutaminase: its utilization in the food industry. Food Rev Int 17:221–246CrossRefGoogle Scholar
  37. Lee M, Lee D, Cho J, Kim S, Park C (2013) Enzymatic biodiesel synthesis in semi-pilot continuous process in near-critical carbon dioxide. Appl Biochem Biotechnol 171:1118–1127CrossRefPubMedGoogle Scholar
  38. Lim TJ, Easa A-M, Karim A-A, Bhat R, Liong M-T (2011) Development of soy-based cream cheese via the addition of microbial transglutaminase, soy protein isolate and maltodextrin. Br Food J 113:1147–1172CrossRefGoogle Scholar
  39. Lv Y, Lin Z, Tan T, Svec F (2014) Preparation of reusable bioreactors using reversible immobilization of enzyme on monolithic porous polymer support with attached gold nanoparticles. Biotechnol Bioeng 111:50–58CrossRefPubMedGoogle Scholar
  40. Mangion IK, Sherry BD, Yin J, Fleitz FJ (2012) Enantioselective synthesis of a dual orexin receptor antagonist. Org Lett 14:3458–3461CrossRefPubMedGoogle Scholar
  41. Markets and Markets (2013) Food enzymes market by types (carbohydrase, protease, lipase), applications (beverages, dairy, bakery), sources (microorganisms, plants, animals), and geography—Global trends and forecasts to, 2018, vol FB 1264. Markets and Markets, DallasGoogle Scholar
  42. Martins IM, Matos M, Costa R, Silva F, Pascoal A, Estevinho LM, Choupina AB (2014) Transglutamineses: recent achievements and new sources. Appl Microbiol Biotechnol 98:6957–6964CrossRefPubMedGoogle Scholar
  43. Mathew S, Shin G, Shon M, Yun H (2013) High throughput screening methods for ω-transaminases. Biotech Bioprocess Eng 18:1–7CrossRefGoogle Scholar
  44. Meadows RE, Mulholland KR, Schurmann M, Golden M, Kierkels H, Meulenbroeks E, Mink D, May O, Squire C, Straatman H, Wells AS (2013) Efficient synthesis of (S)-1-(5-Fluoropyrimidin-2-yl)ethylamine using an omega-transaminase biocatalyst in a two-phase system. Org Process Res Dev 17:1117–1122CrossRefGoogle Scholar
  45. Midelfort KS, Kumar R, Han S, Karmilowicz MJ, McConnell K, Gehlhaar DK, Mistry A, Chang JS, Anderson M, Villalobos A, Minshull J, Govindarajan S, Wong JW (2013) Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin. Protein Eng Des Sel 26:25–33CrossRefPubMedGoogle Scholar
  46. Nestl BM, Nebel BA, Hauer B (2011) Recent progress in industrial biocatalysis. Curr Opin Chem Biol 15:187–193CrossRefPubMedGoogle Scholar
  47. Nestl BM, Hammer SC, Nebel BA, Hauer B (2014) New generation of biocatalysts for organic synthesis. Angew Chem Int Ed Engl 53:3070–3095CrossRefPubMedGoogle Scholar
  48. Ngo TPN, Li A, Tiew KW, Li Z (2013) Efficient transformation of grease to biodiesel using highly active and easily recyclable magnetic nanobiocatalyst aggregates. Bioresour Technol 145:233–239CrossRefPubMedGoogle Scholar
  49. Olivier CE, Lima RP, Pinto DG, Santos RA, Silva GK, Lorena SL, Villas-Boas MB, Netto FM, Zollner Rde L (2012) In search of a tolerance-induction strategy for cow’s milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics 67:1171–1179CrossRefPubMedCentralPubMedGoogle Scholar
  50. Peng X (2013) Improved thermostability of lipase B from Candida antarctica by directed evolution and display on yeast surface. Appl Biochem Biotechnol 169:351–358CrossRefPubMedGoogle Scholar
  51. Rachel NM, Pelletier JN (2013) Biotechnological applications of transglutaminases. Biomolecules 3:870–888CrossRefPubMedCentralPubMedGoogle Scholar
  52. Ramos OS, Malcata FX (2011) Food-grade enzymes. In: Moo-Young M, Butler M, Webb BC et al (eds) Comprehensive Biotechnology. Academic Press, BurlingtonGoogle Scholar
  53. Renzetti S, Behr J, Vogel RF, Barbiroli A, Iametti S, Bonomi F, Arendt EK (2012) Transglutaminase treatment of brown rice flour: a chromatographic, electrophoretic and spectroscopic study of protein modifications. Food Chem 131:1076–1085CrossRefGoogle Scholar
  54. Rouhi AM (2004) Chiral chemistry: traditional methods thrive despite numerous hurdles, including tough luck, slow commercialization of catalytic processes. Chem Eng News 82:47–62CrossRefGoogle Scholar
  55. Rudat J, Brucher BR, Syldatk C (2012) Transaminases for the synthesis of enantiopure beta-amino acids. AMB Express 2:11CrossRefPubMedCentralPubMedGoogle Scholar
  56. Savile CK, Janey JM, Mundorff EC, Moore JC, Tam S, Jarvis WR, Colbeck JC, Krebber A, Fleitz FJ, Brands J, Devine PN, Huisman GW, Hughes GJ (2010) Biocatalytic asymmetric synthesis of chiral amines from ketones applied to sitagliptin manufacture. Science 329:305–309CrossRefPubMedGoogle Scholar
  57. Schoenlechner R, Szatmari M, Bagdi A, Tomoskozi S (2013) Optimisation of bread quality produced from wheat and proso millet (Panicum miliaceum L.) by adding emulsifiers, transglutaminase and xylanase. Lwt-Food Sci Technol 51:361–366CrossRefGoogle Scholar
  58. Seo JH, Kyung D, Joo K, Lee J, Kim BG (2011) Necessary and sufficient conditions for the asymmetric synthesis of chiral amines using omega-aminotransferases. Biotechnol Bioeng 108:253–263CrossRefPubMedGoogle Scholar
  59. Simon RC, Mutti FG, Kroutil W (2013) Biocatalytic synthesis of enantiopure building blocks for pharmaceuticals. Drug Discov Today Technol 10:e37–e44CrossRefPubMedGoogle Scholar
  60. Smerdel B, Pollak L, Novotni D, Cukelj N, Benkovic M, Lusic D, Curic D (2012) Improvement of gluten-free bread quality using transglutaminase, various extruded flours and protein isolates. J Food Nut Res 51:242–253Google Scholar
  61. Stangierski J, Rezler R, Lesnierowski G (2014) Analysis of the effect of heating on rheological attributes of washed mechanically recovered chicken meat modified with transglutaminase. J Food Eng 141:13–19CrossRefGoogle Scholar
  62. Svedendahl M, Branneby C, Lindberg L, Berglund P (2010) Reversed enantiopreference of an omega-transaminase by a single-point mutation. Chemcatchem 2:976–980CrossRefGoogle Scholar
  63. Truppo MD, Rozzell JD, Turner NJ (2010) Efficient production of enantiomerically pure chiral amines at concentrations of 50 g/L using transaminases. Org Process Res Dev 14:234–237CrossRefGoogle Scholar
  64. Truppo MD, Strotman H, Hughes G (2012) Development of an immobilized transaminase capable of operating in organic solvent. ChemCatChem 4:1071–1074CrossRefGoogle Scholar
  65. Tufvesson P, Lima-Ramos J, Jensen JS, Al-Haque N, Neto W, Woodley JM (2011) Process considerations for the asymmetric synthesis of chiral amines using transaminases. Biotechnol Bioeng 108:1479–1493CrossRefPubMedGoogle Scholar
  66. Woodley JM (2013) Protein engineering of enzymes for process applications. Curr Opin Chem Biol 17:310–316CrossRefPubMedGoogle Scholar
  67. Xie Y, An J, Yang G, Wu G, Zhan Y, Cui L, Feng Y (2014) Enhanced enzyme kinetic stability by increasing rigidity within the active site. J Biol Chem 289:7994–8006CrossRefPubMedCentralPubMedGoogle Scholar
  68. Yan J, Zheng X, Du L, Li S (2014) Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnol Biofuels 7:55CrossRefPubMedCentralPubMedGoogle Scholar
  69. Yew SE, Lim TJ, Lew LC, Bhat R, Mat-Easa A, Liong MT (2011) Development of a probiotic delivery system from agrowastes, soy protein isolate, and microbial transglutaminase. J Food Sci 76:H108–H115CrossRefPubMedGoogle Scholar
  70. Yokoyama K, Utsumi H, Nakamura T, Ogaya D, Shimba N, Suzuki E, Taguchi S (2010) Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis. Appl Microbiol Biotechnol 87:2087–2096CrossRefPubMedGoogle Scholar
  71. Yu XW, Tan NJ, Xiao R, Xu Y (2012a) Engineering a disulfide bond in the lid hinge region of Rhizopus chinensis lipase: increased thermostability and altered acyl chain length specificity. Plos one 7:e46388CrossRefPubMedCentralPubMedGoogle Scholar
  72. Yu XW, Wang R, Zhang M, Xu Y, Xiao R (2012b) Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris. Microb Cell Fact 11:1–11CrossRefGoogle Scholar
  73. Zhang D, Zhu Y, Chen J (2009) Microbial transglutaminase production: understanding the mechanism. Biotechnol Gen Eng Rev 26:205–222CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Tanja Narancic
    • 1
  • Reeta Davis
    • 2
  • Jasmina Nikodinovic-Runic
    • 3
  • Kevin E. O’ Connor
    • 1
  1. 1.School of Biomolecular and Biomedical Science, Earth Institute UCDUniversity College DublinDublin 4Ireland
  2. 2.Advanced Materials and BioEngineering ResearchTrinity College DublinDublin 2Ireland
  3. 3.Institute of Molecular Genetics and Genetic EngineeringUniversity of BelgradeBelgradeSerbia

Personalised recommendations