Recent developments in biocatalysis beyond the laboratory
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Recent developments in biocatalysis, where implementation beyond the laboratory has been demonstrated, are explored: the use of transglutaminases to modify foods, reduce allergenicity and produce advanced materials, lipases for biodiesel production, and transaminases for biochemical production. The availability and application of enzymes at pilot and larger scale opens up possibilities for further improvements of biocatalyst-based processes and the development of new processes. Enzyme production, stability, activity, re-use, and product retrieval are common challenges for biocatalytic processes. We explore recent advances in biocatalysis within the process chain, such as protein engineering, enzyme expression, and biocatalyst immobilization, in the context of these challenges.
KeywordsBiocatalysis Enzyme expression Immobilization Lipases Protein engineering Transaminases Transglutaminase
Jasmina Nikodinovic-Runic is partially funded by the Ministry of Education, Science and Technological Development of Serbia Project 173048. Tanja Narancic is funded by the EC FP7 project SYNPOL (311815); Reeta Davis is funded by Science Foundation Ireland AMBER centre (12/RC/2278). Authors acknowledge the Systems Biocatalysis COST Action CM1303.
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