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Biotechnology Letters

, Volume 37, Issue 4, pp 891–898 | Cite as

Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners

  • Mengru Zhang
  • Ming Gong
  • Yumei Yang
  • Xujuan Li
  • Haibo Wang
  • Zhurong ZouEmail author
Original Research Paper

Abstract

Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the level of plant cellular reactive oxygen species and its thermolability is proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion partners, such as the C-terminal peptide tails, were evaluated for their effects on the thermal stability and activity of APX1 from Jatropha curcas and Arabidopsis. The hyper-acidic fusion partners efficiently improved the thermostability and prevented thermal inactivation of APX1 in both plant species with an elevated heat tolerance of at least 2 °C. These hyper-acidified thermostable APX1 fusion variants are of considerable biotechnological potential and can provide a new route to enhance the heat tolerance of plant species especially of inherent thermo-sensitivity.

Keywords

Ascorbate peroxidase 1 Fusion partner Heat-susceptibility Hyper-acidity Jatropha curcas Thermostability 

Notes

Acknowledgments

This work was supported by several grants from the National Foundations of Natural Sciences, China (No. 31260064 to M. Gong, No. 31060160, 31160169 to Z. Zou).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mengru Zhang
    • 1
  • Ming Gong
    • 1
  • Yumei Yang
    • 1
  • Xujuan Li
    • 2
  • Haibo Wang
    • 3
  • Zhurong Zou
    • 1
    Email author
  1. 1.Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life SciencesYunnan Normal UniversityKunmingChina
  2. 2.Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research InstituteYunnan Academy of Agricultural SciencesKaiyuanChina
  3. 3.College of Biological Resources and Environmental ScienceQujing Normal UniversityQujingChina

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