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Biotechnology Letters

, Volume 37, Issue 4, pp 935–942 | Cite as

Fibronectin immobilization on to robotic-dispensed nanobioactive glass/polycaprolactone scaffolds for bone tissue engineering

  • Jong-Eun Won
  • Miguel A. Mateos-Timoneda
  • Oscar Castano
  • Josep A. Planell
  • Seog-Jin Seo
  • Eun-Jung Lee
  • Cheol-Min Han
  • Hae-Won KimEmail author
Original Research Paper

Abstract

Bioactive nanocomposite scaffolds with cell-adhesive surface have excellent bone regeneration capacities. Fibronectin (FN)-immobilized nanobioactive glass (nBG)/polycaprolactone (PCL) (FN-nBG/PCL) scaffolds with an open pore architecture were generated by a robotic-dispensing technique. The surface immobilization level of FN was significantly higher on the nBG/PCL scaffolds than on the PCL scaffolds, mainly due to the incorporated nBG that provided hydrophilic chemical-linking sites. FN-nBG/PCL scaffolds significantly improved cell responses, including initial anchorage and subsequent cell proliferation. Although further in-depth studies on cell differentiation and the in vivo animal responses are required, bioactive nanocomposite scaffolds with cell-favoring surface are considered to provide promising three-dimensional substrate for bone regeneration.

Keywords

Bone scaffolds Cell response Fibronectin Nanobioactive glass Nanocomposites Polycaprolactone 

Notes

Acknowledgments

This work was supported by a Grant of Priority Research Centers Program (Grant #2009-0093829), through the National Research Foundation of Korea (NRF), Republic of Korea.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jong-Eun Won
    • 1
    • 2
  • Miguel A. Mateos-Timoneda
    • 3
    • 4
  • Oscar Castano
    • 4
  • Josep A. Planell
    • 4
  • Seog-Jin Seo
    • 1
    • 2
  • Eun-Jung Lee
    • 1
    • 2
  • Cheol-Min Han
    • 1
    • 5
  • Hae-Won Kim
    • 1
    • 2
    • 5
    Email author
  1. 1.Institute of Tissue Regeneration Engineering (ITREN)Dankook UniversityCheonanSouth Korea
  2. 2.Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative MedicineDankook UniversityCheonanSouth Korea
  3. 3.Biomaterials for Regenerative TherapiesInstitute for Bioengineering of Catalonia (IBEC)BarcelonaSpain
  4. 4.CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN)BarcelonaSpain
  5. 5.Department of Biomaterials Science, College of DentistryDankook UniversityCheonanSouth Korea

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