Biotechnology Letters

, Volume 37, Issue 3, pp 533–538 | Cite as

Culturing Drosophila melanogaster (S2) in a chemostat

  • Paula Bruzadelle Vieira
  • Bruno Labate Vale da Costa
  • Elisabeth de Fatima Pires Augusto
  • Aldo Tonso
Original Research Paper

Abstract

Insect cells are used for the expression of complex proteins in products such as vaccines and biopharmaceuticals. Physiology of a Drosophila melanogaster (lineage S2), transfected to stably express rabies virus glycoprotein (RVGP), was studied in batch culture and in a chemostat with serum-free medium. In batch mode, the system reached 3 × 107 cells mL−1 with specific growth rate of 1.5 d−1 with RVGP at 2.50 µg L−1. When operated continuously, three well-defined steady states were achieved at dilution rates (D) of 0.8, 0.5 and 0.2 d−1. The residual glucose and glutamine concentrations and the cell yields on glucose and glutamine decreased at lower D. High values of substrate consumption for maintenance may explain this variation in yields. The results indicated that glucose is not the limiting substrate of this process.

Keywords

Bioprocess engineering Bioreactor Continuous culture Drosophila melanogaster Insect cell Rabies virus glycoprotein 

List of abbreviations

CV

Coefficient of variation

D

Dilution rate

GLC

Glucose concentration

GLN

Glutamine concentration

mS

Maintenance coefficient

µMax

Maximum specific growth rate

NH4

Ammonium concentration

RVGP

Rabies virus glycoprotein

S2

Drosophila melanogaster cells lineage

X

Viable cell concentration

YNH4/GLN

Ammonium yield on glutamine

YNH4/X

Ammonium yield on cells

YX/GLC

Cell yield on glucose

YX/GLN

Cell yield on glutamine

YX/S

Global cell yield on substrate

YX/Strue

True cell yield on substrate

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Paula Bruzadelle Vieira
    • 1
  • Bruno Labate Vale da Costa
    • 1
  • Elisabeth de Fatima Pires Augusto
    • 2
  • Aldo Tonso
    • 1
  1. 1.Laboratório de Células AnimaisEscola Politécnica––Universidade de São PauloSão PauloBrazil
  2. 2.Universidade Federal de São Paulo––Campus São José dos CamposSão José dos CamposBrazil

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