Biotechnology Letters

, Volume 37, Issue 2, pp 265–279 | Cite as

Recent developments in therapeutic protein expression technologies in plants

  • Shah Fahad
  • Faheem Ahmed Khan
  • Nuruliarizki Shinta Pandupuspitasari
  • Muhammad Mahmood Ahmed
  • Yu Cai Liao
  • Muhammad Tahir Waheed
  • Muhammad Sameeullah
  • Darkhshan
  • Saddam Hussain
  • Shah Saud
  • Shah Hassan
  • Amanullah Jan
  • Mohammad Tariq Jan
  • Chao Wu
  • Ma Xiao Chun
  • Jianliang Huang


Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.


Antibodies Bacterial cells Biopharmaceuticals Mammalian cells Protein expression Vaccines 



We thank the Key Technology Program R & D of China (project no. 2012BAD04B12) and MOA Special Fund for Agro-Scientific Research in the Public Interest of China (no. 201103003) for funding.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Shah Fahad
    • 1
  • Faheem Ahmed Khan
    • 2
    • 3
  • Nuruliarizki Shinta Pandupuspitasari
    • 3
  • Muhammad Mahmood Ahmed
    • 4
  • Yu Cai Liao
    • 4
  • Muhammad Tahir Waheed
    • 5
  • Muhammad Sameeullah
    • 8
  • Darkhshan
    • 9
  • Saddam Hussain
    • 1
  • Shah Saud
    • 6
  • Shah Hassan
    • 7
  • Amanullah Jan
    • 7
  • Mohammad Tariq Jan
    • 7
  • Chao Wu
    • 1
  • Ma Xiao Chun
    • 1
  • Jianliang Huang
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.Molecular Biotechnology Laboratory for Triticeae CropsHuazhong Agricultural UniversityWuhanChina
  3. 3.Key Laboratory of Agricultural Animal Genetics, Breeding and ReproductionHuazhong Agricultural UniversityWuhanChina
  4. 4.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  5. 5.Department of BiochemistryQuaid-I-Azam UniversityIslamabadPakistan
  6. 6.Department of HorticulturalNortheast Agricultural UniversityHarbinChina
  7. 7.Agriculture UniversityPeshawarPakistan
  8. 8.Biotechnology Lab., Department of Biology, Faculty of Science and ArtsAbant Izzet Baysal UniversityBoluTurkey
  9. 9.Women Institute of LearningAbbottabadPakistan

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