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Biotechnology Letters

, Volume 37, Issue 2, pp 359–366 | Cite as

Enhanced photo-H2 production by unsaturated flow condition in continuous culture

  • Cheng-Long GuoEmail author
  • Hong-Xia Cao
  • Fei-Qiang Guo
  • Cong-Liang Huang
  • Huan-Guang Wang
  • Zhong-Hao RaoEmail author
Original Research Paper

Abstract

A biofilm photobioreactor under unsaturated flow condition (BFPBR-U) is proposed using a polished optical fiber as the internal light source for photo-H2 production in continuous culture. The main chamber was filled with spherical glass beads to create the reaction bed and the cells were immobilized to form a biofilm under unsaturated flow condition obtained by pumping substrate solution over a packing bed at a rate to create a thin fluid film and injecting the argon to maintain the gas phase space. The effects of operational conditions, including flow rate and influent substrate concentration, on the photo-H2 production performance were investigated. The unsaturated flow conditions eliminated the inhibition caused by high organic loading rate and enhanced light transmission efficiency, leading to an improvement in the photo-H2 production performance.

Keywords

Biofilm photobioreactor H2 production Optical fiber Photo--H2 production Photosynthetic bacteria Unsaturated flow conditions 

Notes

Acknowledgments

The authors gratefully acknowledge financial supported by the Fundamental Research Funds for the Central Universities (No. 2014QNB03), the National Natural Science Foundation of China (No. 51406227) and sincerely appreciate Prof. Qiang Liao and Prof. Xun Zhu from Chongqing University for their suggestion on the experiment.

References

  1. Bai MD, Chao YC, Lin YH, Lu WC, Lee HT (2009) Immobilized biofilm used as seeding source in batch biohydrogen fermentation. Renew Energy 34:1969–1972CrossRefGoogle Scholar
  2. Chen CY, Lee CM, Chang JS (2006) Feasibility study on bioreactor strategies for enhanced photo-H2 production from Rhodopseudomonas palustris WP3-5 using optical-fiber-assisted illumination systems. Int J Hydrog Energy 31:2345–2355CrossRefGoogle Scholar
  3. Guo CL, Zhu X, Liao Q, Wang YZ, Chen R, Lee DJ (2011) Enhancement of photo-H2 production in a biofilm photobioreactor using optical fiber with additional rough surface. Bioresour Technol 102:8507–8513PubMedCrossRefGoogle Scholar
  4. Hallenbeck PC, Benemann JR (2002) Biological H2 production; fundamentals and limiting processes. Int J Hydrog Energy 27:1185–1193CrossRefGoogle Scholar
  5. Liao Q, Wang YJ, Wang YZ, Zhu X, Tian X, Li J (2010) Formation and H2 production of photosynthetic bacterial biofilm under various illumination conditions. Bioresour Technol 101:5315–5324PubMedCrossRefGoogle Scholar
  6. Miyake J, Miyake M, Asada Y (1999) Biotechnological H2 production: research for efficient light energy conversion. J Biotechnol 70:89–101CrossRefGoogle Scholar
  7. Redwood MD, Dhillon R, Orozco RL, Zhang X, Binks DJ, Dickinson M, Macaskie LE (2012) Enhanced photosynthetic output via dichroic beam-sharing. Biotechnol Lett 34:2229–2234PubMedCentralPubMedCrossRefGoogle Scholar
  8. Su H, Cheng J, Zhou J, Song W, Cen K (2009) Combination of dark- and photo-fermentation to enhance H2 production and energy conversion efficiency. Int J Hydrog Energy 34:8846–8853CrossRefGoogle Scholar
  9. Tian X, Liao Q, Zhu X, Wang YZ, Zhang P, Li J, Wang H (2010) Characteristics of a biofilm photobioreactor as applied to photo-H2 production. Bioresour Technol 101:977–983PubMedCrossRefGoogle Scholar
  10. Wang YZ, Liao Q, Zhu X, Tian X, Zhang C (2010) Characteristics of H2 production and substrate consumption of Rhodopseudomonas palustris CQK 01 in an immobilized-cell photobioreactor. Bioresour Technol 101:4034–4041PubMedCrossRefGoogle Scholar
  11. Wang YZ, Liao Q, Zhu X, Chen R, Guo CL, Zhou J (2013) Bioconversion characteristics of Rhodopseudomonas palustris CQK 01 entrapped in a photobioreactor for H2 production. Bioresour Technol 135:331–338PubMedCrossRefGoogle Scholar
  12. Zhang HS, Bruns MA, Logan BE (2006) Biological H2 production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res 40:728–734PubMedCrossRefGoogle Scholar
  13. Zhang C, Zhu X, Liao Q, Wang YZ, Li J, Ding YD, Wang H (2010) Performance of a groove-type photobioreactor for H2 production by immobilized photosynthetic bacteria. Int J Hydrog Energy 35:5284–5292CrossRefGoogle Scholar
  14. Zhang C, Wang AJ, Zhang QG (2013) A two-dimensional mass transfer model for an annular bioreactor using immobilized photosynthetic bacteria for H2 production. Biotechnol Lett 35:1579–1587PubMedCrossRefGoogle Scholar
  15. Zhu X, Xie XW, Liao Q, Wang YZ, Lee DJ (2011) Enhanced H2 production by Rhodopseudomonas palustris CQK 01 with ultra-sonication pretreatment in batch culture. Bioresour Technol 102:8696–8699PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Cheng-Long Guo
    • 1
    • 2
    Email author
  • Hong-Xia Cao
    • 3
  • Fei-Qiang Guo
    • 1
  • Cong-Liang Huang
    • 1
  • Huan-Guang Wang
    • 1
  • Zhong-Hao Rao
    • 1
    Email author
  1. 1.School of Electric Power EngineeringChina University of Mining and TechnologyXuzhouChina
  2. 2.Institute of Engineering ThermophysicsChongqing UniversityChongqingChina
  3. 3.Low Carbon Energy InstituteChina University of Mining and TechnologyXuzhouChina

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