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Biotechnology Letters

, Volume 36, Issue 11, pp 2319–2324 | Cite as

Enhanced production of poly-γ-glutamic acid by a newly-isolated Bacillus subtilis

  • Wan-Taek Ju
  • Yong-Su Song
  • Woo-Jin Jung
  • Ro-Dong Park
Original Research Paper

Abstract

Application of poly-gamma-glutamic acid (γ-PGA), an unusual macromolecular anionic polypeptide, is limited due to the high cost associated with its low productivity. Screening bacterial strains to find a more efficient producer is one approach to overcome this limitation. Strain MJ80 was isolated as a γ-PGA producer among 1,500 bacterial colonies obtained from soil samples. It was identified as Bacillus subtilis, based on the biochemical and morphological properties and 16S rDNA gene sequencing. It produced γ-PGA from both glutamic acid and soybean powder, identifying it as a facultative glutamic acid-metabolizing bacterium. After optimization of its culture conditions, B. subtilis MJ80 showed γ-PGA productivity of 75.5 and 68.7 g/l in 3 and 300 l jar fermenters for 3 days cultivation, respectively, the highest productivity reported to date, suggesting MJ80 to be a promising strain for γ-PGA production.

Keywords

Anionic polypeptide Bacillus subtilis MJ80 Glutamic acid γ-PGA Poly-γ-glutamic acid 

Notes

Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (No.PJ007983), Rural Development Administration (RDA), Republic of Korea.

Supplementary material

10529_2014_1613_MOESM1_ESM.docx (168 kb)
Supplementary material 1 (DOCX 167 kb)

References

  1. Bajaj IB, Singhal RS (2011) Poly(glutamic acid)—an emerging biopolymer of commercial interest. Bioresour Technol 102:5551–5561PubMedCrossRefGoogle Scholar
  2. Cao M, Geng W, Song C, Xie H, Guo W, Jin Y, Wang S (2011) Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresour Technol 102:4251–4257PubMedCrossRefGoogle Scholar
  3. Cromwick AM, Birrer GA, Gross RA (1996) Effects of pH and aeration on γ-poly(glutamic acid) formation by Bacillus licheniformis in controlled batch fermentor cultures. Biotechnol Bioeng 50:222–227PubMedCrossRefGoogle Scholar
  4. Du G, Yang G, Qu Y, Chen J, Lun S (2005) Effects of glycerol on the production of poly(γ-glutamic acid) by Bacillus licheniformis. Proc Biochem 40:2143–2147CrossRefGoogle Scholar
  5. Ito Y, Tanaka T, Ohmachi T, Asada Y (1996) Glutamic acid independent production of poly(γ-glutamic acid) by Bacillus subtilis TAM-4. Biosci Biotechnol Biochem 60:1239–1242CrossRefGoogle Scholar
  6. Jeong JH, Kim JN, Wee YJ, Ryu HW (2010) The statistically optimized production of poly (γ-glutamic acid) by batch fermentation of a newly isolated Bacillus subtilis RKY3. Bioresour Technol 101:4533–4539PubMedCrossRefGoogle Scholar
  7. Kang HS, Park SH, Lee YZ, Son TI (2007) Polyelectrohydrate complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: preparation, physical properties, and cytocompatibility. J Appl Polym Sci 103:386–394CrossRefGoogle Scholar
  8. Kubota H, Matsunobu T, Uotani K, Takebe H, Satoh A, Tanaka T (1993) Production of poly(γ-glutamic acid) by Bacillus subtilis F-2-01. Biosci Biotechnol Biochem 57:1212–1213CrossRefGoogle Scholar
  9. Kunioka M, Goto A (1994) Biosynthesis of poly(γ-glutamic acid) from l-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335. Appl Microbiol Biotechnol 40:867–872CrossRefGoogle Scholar
  10. Lin YH, Chung CK, Chen CT, Liang HF, Chen SC, Sung HW (2005) Preparation of nanoparticles composed of chitosan/polygamma-glutamic acid and evaluation of their permeability through caco-2 cells. Biomacromolecules 6:1104–1112PubMedCrossRefGoogle Scholar
  11. Ogawa Y, Yamaguchi F, Yuasa K, Tahara Y (1997) Efficient production γ-polyglutamic acid by Bacillus subtilis (natto) in jar fermenters. Biosci Biotechnol Biochem 61:1684–1687CrossRefGoogle Scholar
  12. Park C, Choi JC, Choi YH, Nakamura H, Shimanouchi K, Horiuchi T, Misono H, Sewaki T, Soda K, Ashiuchi M, Sung MH (2005) Synthesis of super-highmolecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. Chungkookjang. J Mol Catal B 35:128–133CrossRefGoogle Scholar
  13. Qiao C, Zhang S, Li Z, Chen X, Li X, Lan L (2013) Improving poly-(γ-glutamic acid) production and reducing impurities in fermentation broth by medium optimization using Bacillus licheniformis CGMCC3336. J Biobased Mater Bioenergy 7:390–394CrossRefGoogle Scholar
  14. Shih IL, Van YT (2001) The production of poly(γ-glutamic acid) from microorganism and its various applications. Bioresour Technol 79:207–225PubMedCrossRefGoogle Scholar
  15. Sung M, Park C, Kim C, Poo H, Soda K, Ashiuchi M (2005) Natural and edible biopolymer poly-γ-glutamic acid: synthesis, production, and application. Chem Rec 5:352–366PubMedCrossRefGoogle Scholar
  16. Wu Q, Xu H, Liang J, Yao J (2010) Contribution of glycerol on production of poly(γ-glutamic acid) in B. subtilis NX-2. Appl Biochem Biotechnol 160:386–392PubMedCrossRefGoogle Scholar
  17. Yoon SH, Do JH, Lee SY, Chang HN (2000) Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheniformis. Biotechnol Lett 22:585–588CrossRefGoogle Scholar
  18. Zeng W, Lin Y, Qi Z, He Y, Wang D, Chen G, Liang Z (2013) An integrated high-throughput strategy for rapid screening of poly(γ-glutamic acid)-producing bacteria. Appl Microbiol Biotechnol 97:2163–2172PubMedCrossRefGoogle Scholar
  19. Zhang D, Feng X, Li ZS, Chen F, Xu H (2012) Effects of oxygen vectors on the synthesis and molecular weight of poly(γ-glutamic acid) and the metabolic characterization of Bacillus subtilis NX-2. Proc Biochem 47:2103–2109CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Wan-Taek Ju
    • 1
  • Yong-Su Song
    • 1
  • Woo-Jin Jung
    • 1
  • Ro-Dong Park
    • 1
  1. 1.Division of Applied Bioscience and Biotechnology, Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Institute of Agricultural Science and TechnologyChonnam National UniversityGwangjuRepublic of Korea

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