Biotechnology Letters

, Volume 36, Issue 11, pp 2319–2324 | Cite as

Enhanced production of poly-γ-glutamic acid by a newly-isolated Bacillus subtilis

  • Wan-Taek Ju
  • Yong-Su Song
  • Woo-Jin Jung
  • Ro-Dong Park
Original Research Paper


Application of poly-gamma-glutamic acid (γ-PGA), an unusual macromolecular anionic polypeptide, is limited due to the high cost associated with its low productivity. Screening bacterial strains to find a more efficient producer is one approach to overcome this limitation. Strain MJ80 was isolated as a γ-PGA producer among 1,500 bacterial colonies obtained from soil samples. It was identified as Bacillus subtilis, based on the biochemical and morphological properties and 16S rDNA gene sequencing. It produced γ-PGA from both glutamic acid and soybean powder, identifying it as a facultative glutamic acid-metabolizing bacterium. After optimization of its culture conditions, B. subtilis MJ80 showed γ-PGA productivity of 75.5 and 68.7 g/l in 3 and 300 l jar fermenters for 3 days cultivation, respectively, the highest productivity reported to date, suggesting MJ80 to be a promising strain for γ-PGA production.


Anionic polypeptide Bacillus subtilis MJ80 Glutamic acid γ-PGA Poly-γ-glutamic acid 



This work was supported by a grant from the Next-Generation BioGreen 21 Program (No.PJ007983), Rural Development Administration (RDA), Republic of Korea.

Supplementary material

10529_2014_1613_MOESM1_ESM.docx (168 kb)
Supplementary material 1 (DOCX 167 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Wan-Taek Ju
    • 1
  • Yong-Su Song
    • 1
  • Woo-Jin Jung
    • 1
  • Ro-Dong Park
    • 1
  1. 1.Division of Applied Bioscience and Biotechnology, Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Institute of Agricultural Science and TechnologyChonnam National UniversityGwangjuRepublic of Korea

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