Biotechnological approach towards a highly efficient production of natural prostaglandins
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Prostaglandins (PGs) act as potent local hormones in nearly all tissues of the human body and are used for various medical applications. Heterologous expression of PG endoperoxide H-synthase from the alga, Gracilaria vermiculophylla, into E. coli and the application of this strain in biotransformation experiments resulted in a highly efficient conversion of arachidonic acid (ARA) yielding up to 130 mg natural PGs l−1 in a laboratory scale approach. Detailed analyses of the products and production kinetics were performed, confirming a rapid conversion of ARA to PGs.
KeywordsArachidonic acid Biotransformation E. coli Gracilaria vermiculophylla Prostaglandin Prostaglandin endoperoxide H-synthase
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