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Biotechnology Letters

, Volume 36, Issue 11, pp 2331–2341 | Cite as

Overexpression of an alfalfa GDP-mannose 3, 5-epimerase gene enhances acid, drought and salt tolerance in transgenic Arabidopsis by increasing ascorbate accumulation

  • Lichao Ma
  • Yanrong Wang
  • Wenxian Liu
  • Zhipeng LiuEmail author
Original Research Paper

Abstract

GDP-mannose 3′, 5′-epimerase (GME) catalyses the conversion of GDP-d-mannose to GDP-l-galactose, an important step in the ascorbic acid (ascorbic acid) biosynthetic pathway in higher plants. In this study, a novel cDNA fragment (MsGME) encoding a GME protein was isolated and characterised from alfalfa (Medicago sativa). An expression analysis confirmed that MsGME expression was induced by salinity, PEG and acidity stresses. MsGME overexpression in Arabidopsis enhanced tolerance of the transgenic plants to salt, drought and acid. Real-time PCR analysis revealed that the transcript levels of GDP-d-mannose pyrophosphorylase (GMP), l-galactose-phosphate 1-P phosphatase (GP) and GDP-l-galactose phosphorylase (GGP) were increased in transgenic Arabidopsis (T3 generation). Moreover, the ascorbate content was increased in transgenic Arabidopsis. Our results suggest that MsGME can effectively enhance tolerance of transgenic Arabidopsis to acid, drought and salt by increasing ascorbate accumulation.

Keywords

Acid tolerance Alfalfa Arabidopsis Ascorbic acid Drought tolerance GDP-mannose 3′, 5′-epimerase Salt tolerance Transgenic plants 

Notes

Acknowledgments

This study was supported by the National Basic Research Program of China (2014CB138704) and the National Natural Science Foundation of China (31072072).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Lichao Ma
    • 1
    • 2
  • Yanrong Wang
    • 1
  • Wenxian Liu
    • 1
  • Zhipeng Liu
    • 1
    Email author
  1. 1.State Key Laboratory of Grassland Agro-ecosystems, School of Pastoral Agricultural Science and TechnologyLanzhou UniversityLanzhouPeople’s Republic of China
  2. 2.Graduate Schoo1 of Lanzhou UniversityLanzhouPeople’s Republic of China

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