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Biotechnology Letters

, Volume 36, Issue 11, pp 2229–2237 | Cite as

Characterization of industrially-valuable xylano-pectinolytic enzymes produced concurrently by a novel isolate of Bacillus pumilus

  • Amanjot Kaur
  • Avtar Singh
  • Ritu MahajanEmail author
Original Research Paper

Abstract

Xylanase and polygalacturonase were concurrently produced by a novel alkalo-thermotolerant Bacillus pumilus AJK. They were purified and characterized to evaluate their potential for various industrial applications. Xylanase was purified to 19-fold with 67 % recovery and polygalacturonase up to 23-fold with 75 % recovery. Existence of multiple forms of xylanase was indicated by its elution-profile through Sephadex G-100 as two peaks, xylanase-I and xylanase-II, with molecular weights of ~24.5 and ~13 kDa, respectively, and by the presence of two pH optima, one at pH 6.0 and other at pH 8.5. The molecular weight of polygalacturonase was ~40 kDa by gel-filtration chromatography. Zymographic studies confirmed the presence of seven isozymic forms of xylanase. Xylanase and polygalacturonase are stable over a broad range of pH and temperature.

Keywords

Bacillus pumilus Pectinase Polygalacturonase Purification Ultrafiltration Xylanase Xylano-pectinolytic enzyme Zymography 

Notes

Acknowledgments

The authors acknowledge the financial support provided by University Grant Commission, India. University Research Scholarship awarded to Amanjot Kaur by Kurukshetra University is also kindly acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia

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