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Biotechnology Letters

, Volume 36, Issue 7, pp 1503–1513 | Cite as

Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum

  • Alexander Berrocal
  • Claudia Oviedo
  • Kenneth W. Nickerson
  • José Navarrete
Original Research Paper

Abstract

Quorum sensing (QS) activity in Ophiostoma fungi has not been described. We have examined the growth conditions on the control of dimorphism in Ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with QS activity. In a defined culture medium with l-proline as the N source, a high inoculum size (107 c.f.u. ml−1) was the principal factor that promoted yeast-like growth. Inoculum size effect can be explained by the secretion of a QS molecule(s) (QSMs) responsible for inducing yeast morphology. QSM candidates were extracted from spent medium and their structure was determined by GC–MS. Three cyclic sesquiterpenes were found. The most abundant molecule, and therefore the principal candidate to be the QSM responsible for yeast growth of O. floccosum, was 1,1,4a-trimethyl-5,6-dimethylene-decalin (C15H24). Other two compounds were also detected.

Keywords

Cyclic sesquiterpenes Fungal dimorphism Inoculum size Ophiostoma floccosum Quorum sensing molecules Trimethyl-dimethylene-decalin 

Notes

Acknowledgments

The principal author would like to thank the personal of the Laboratory of Biodegradation in the Department of Wood Engineering at the University of Bio–Bio (Chile) for all support. To the Graduate School and Department of Wood Engineering at the University of Bio–Bio, the OEA Scholarship Program and the Scholarship Program of the Technology Institute of Costa Rica for providing the funds to carry out this research. We also thank Ph.D. Juanita Freer and Susana Casas from the Laboratory of Renewable Resources at the University of Concepcion for providing the facilities for GC–MS analyses.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Alexander Berrocal
    • 1
  • Claudia Oviedo
    • 2
  • Kenneth W. Nickerson
    • 3
  • José Navarrete
    • 4
  1. 1.Escuela de Ingeniería ForestalInstituto Tecnológico de Costa RicaCartagoCosta Rica
  2. 2.Departamento de Química, Facultad de CienciasUniversidad del Bío BíoConcepciónChile
  3. 3.School of Biological SciencesUniversity of NebraskaLincolnUSA
  4. 4.Departamento de Ingeniería en Maderas, Facultad de IngenieríaUniversidad del Bío BíoConcepciónChile

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