Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum
- 441 Downloads
Quorum sensing (QS) activity in Ophiostoma fungi has not been described. We have examined the growth conditions on the control of dimorphism in Ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with QS activity. In a defined culture medium with l-proline as the N source, a high inoculum size (107 c.f.u. ml−1) was the principal factor that promoted yeast-like growth. Inoculum size effect can be explained by the secretion of a QS molecule(s) (QSMs) responsible for inducing yeast morphology. QSM candidates were extracted from spent medium and their structure was determined by GC–MS. Three cyclic sesquiterpenes were found. The most abundant molecule, and therefore the principal candidate to be the QSM responsible for yeast growth of O. floccosum, was 1,1,4a-trimethyl-5,6-dimethylene-decalin (C15H24). Other two compounds were also detected.
KeywordsCyclic sesquiterpenes Fungal dimorphism Inoculum size Ophiostoma floccosum Quorum sensing molecules Trimethyl-dimethylene-decalin
The principal author would like to thank the personal of the Laboratory of Biodegradation in the Department of Wood Engineering at the University of Bio–Bio (Chile) for all support. To the Graduate School and Department of Wood Engineering at the University of Bio–Bio, the OEA Scholarship Program and the Scholarship Program of the Technology Institute of Costa Rica for providing the funds to carry out this research. We also thank Ph.D. Juanita Freer and Susana Casas from the Laboratory of Renewable Resources at the University of Concepcion for providing the facilities for GC–MS analyses.
- Abbassy MA, Marei GI (2013) Antifungal and chemical composition of essential oils of Juniperus communis L. and Thymus vulgaris L. against two phytopathogenic fungi. J Appl Sci Res 9:4584–4588Google Scholar
- Bisht A, Bisht GRS, Singh M, Gupta R, Singh V (2011) Chemical compsition and antimicrobial activity of essential oil of tubers of Cyperus rotundus Linn. collected from Dehradun (Uttarakhand). J Pharm Biomed Sci 2:661–665Google Scholar
- Hanssen HP (1993) Volatile metabolites produced by species of Ophiostoma and Ceratocystis. In: Wingfield MJ, Seifert KA, Webber JF (eds) Ceratocystis and ophiostoma: taxonomy ecology and pathogenicity. APS Press, St. Paul, pp 117–125Google Scholar
- Karamian R, Asadbegy M, Pakzad R (2013) Essential oil compositions and in vitro antioxidant and antibacterial activities of the methanol extracts of two Salvia species (Lamiaceae) from Iran. Intl J Agri Crop Sci 5:1171–1182Google Scholar
- Muthukamar G, Nickerson KW (1984) Ca(II)-Calmodulin regulation of fungal dimorphism in Ceratocystis ulmi. J Bacteriol 159:390–392Google Scholar
- Pednekar PP, Vakil BV, Sane RT, Datar AG (2013) Phytochemical profile and antioxidant activity of the essential oil from Blumea eriantha DC. Int J Pharm Sci 5:404–413Google Scholar
- Sarsenbayev KN, Sarsenbayeva AB, Balmukanov KU (2013) chemical composition of low-molecular weight organic compounds (LMWOC) of water extracts from Cistanche Deserticola stolones depending on treatment. World Appl Sci J 25:28–35Google Scholar
- Shafaghat A (2009) Composition and antibacterial activity of the volatile oils from different parts of Achillea tenuifolia Lam. from Iran. J Med Plants Res 8:93–98Google Scholar
- Zeringue HJ Jr, Bhatnagar D, Cleveland TE (1993) C15H24 volatile compounds unique to aflatoxigenic strains of Aspergillus flavus. Appl Environ Microb 59:2264–2270Google Scholar