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Biotechnology Letters

, Volume 36, Issue 5, pp 947–960 | Cite as

A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells

  • Ailing Teo
  • Athanasios Mantalaris
  • Kedong Song
  • Mayasari LimEmail author
Original Research Paper

Abstract

Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 105 cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.

Keywords

Bioreactors Cardiac tissue engineering Embryonic stem cells Stem cell differentiation Three-dimensional culture Tissue engineering Troponin 

Abbreviation

HARV

High-aspect rotating vessel

List of symbols

ρ

Density of fluid

υ

Viscosity of fluid

Dmi

Diffusion coefficient of glucose

Yi

Mass fraction of glucose

Ji

Diffusion flux of glucose

Ri

Net production rate of glucose

Si

User defined source from neighboring grids of glucose

Notes

Acknowledgments

The authors would like to acknowledge the financial support provided by Nanyang Technological University (NTU)-Imperial joint PhD program and Singapore National Research Foundation (NRF) under the NRF-Technion Research Thrust (NRF-Technion/2012-SG#04).

Supplementary material

10529_2014_1456_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Ailing Teo
    • 1
    • 2
  • Athanasios Mantalaris
    • 2
  • Kedong Song
    • 3
  • Mayasari Lim
    • 1
    • 4
    Email author
  1. 1.School of Chemical & Biomedical EngineeringNanyang Technological UniversitySingaporeSingapore
  2. 2.Department of Chemical EngineeringImperial College LondonLondonUK
  3. 3.School of Chemical Engineering & TechnologyDalian University of TechnologyDalianChina
  4. 4.Division of BioengineeringNanyang Technological UniversitySingaporeSingapore

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