A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells
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Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 105 cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.
KeywordsBioreactors Cardiac tissue engineering Embryonic stem cells Stem cell differentiation Three-dimensional culture Tissue engineering Troponin
High-aspect rotating vessel
List of symbols
Density of fluid
Viscosity of fluid
Diffusion coefficient of glucose
Mass fraction of glucose
Diffusion flux of glucose
Net production rate of glucose
User defined source from neighboring grids of glucose
The authors would like to acknowledge the financial support provided by Nanyang Technological University (NTU)-Imperial joint PhD program and Singapore National Research Foundation (NRF) under the NRF-Technion Research Thrust (NRF-Technion/2012-SG#04).
- Hwang YS, Randle WL, Bielby RC, Polak JM, Mantalaris A (2006) Enhanced derivation of osteogenic cells from murine embryonic stem cells after treatment with HepG2-conditioned medium and modulation of the embryoid body formation period: application to skeletal tissue engineering. Tissue Eng 12:1381–1392PubMedCrossRefGoogle Scholar