Encapsulation and crystallization of Prussian blue nanoparticles by cowpea chlorotic mottle virus capsids
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Cowpea chlorotic mottle virus (CCMV) capsids were used to encapsulate Prussian blue (PB) particles based on electrostatic interaction. A negatively-charged metal complex, hexacyanoferrate (III), was entrapped inside the capsids through the disassembly/reassembly process under a pH change from 7.5 to 5.2. The loaded capsids reacted with a second Fe(II) to fabricate PB particles. The synthesis of PB in CCMV capsids was confirmed by a unique colour transition at 710 nm and by size-exclusion FPLC. Transmission electron microscopy images of PB-CCMV biohybrids presented discrete spherical particles with a relatively homogeneous size. Dynamic light scattering of PB-CCMV showed two peaks of 29.2 ± 1.7 nm corresponding to triangulation number T = 3 particles, and 17.5 ± 1.2 nm of pseudo T = 2 particles. The encapsulation and crystallization of PB in CCMV provided an efficient method for the self-organization of bimetallic nanoparticles.
KeywordsBiohybrid Bimetallic nanoparticles Capsids Cowpea chlorotic mottle virus Encapsulation Nanoparticles Prussian blue
We thank Dr. Young-Chul Lee (KAIST) for providing the TEM pictures. This study was supported by the research fund from Chosun University, 2013.
- Chen Z, Stauffacher C, Johnson JE (1990) Capsid structure and RNA packaging in comovirus. Semin Virol 1:453–466Google Scholar
- Sheehan D, O’Sullivan S (2003) Fast protein liquid chromatography. Protein purification protocols 244Google Scholar
- Wu Y, Yang H, Shin HJ (2011) Expression and self assembly of cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and encapsulation of fluorescent myoglobin. MRS Proceedings 1317:mrsf10-1317-rr03-05 doi: 10.1557/opl.2011.138