Biotechnology Letters

, Volume 36, Issue 3, pp 667–676

Human adipose-derived stem cells modified by HIF-1α accelerate the recovery of cisplatin-induced acute renal injury in vitro

  • WeiWei Wang
  • Wei Wang
  • Yan Jiang
  • Zezheng Li
  • Jin Cheng
  • Nanmei Liu
  • GuoFeng Han
  • Shi Lu
  • JinYuan Zhang
Original Research Paper

DOI: 10.1007/s10529-013-1389-x

Cite this article as:
Wang, W., Wang, W., Jiang, Y. et al. Biotechnol Lett (2014) 36: 667. doi:10.1007/s10529-013-1389-x

Abstract

Human adipose-derived stem cells (hASCs) improve renal function in acute kidney injury. Hypoxia-inducible factor-1α (HIF-1α) was transfected into hASCs. hASCs modified by lentivirus-mediated empty-vector and HIF-1α maintained their stem cell characteristics. The expression of the renal-protective gene, heme oxygenase-1 and vascular endothelial growth factor were significantly increased in hASCs modified by HIF-1α, compared to hASCs modified by empty-vector. Cellular ultra-structure and TUNEL staining revealed that hASCs modified by HIF-1α promoted the recovery of apoptotic morphology in cisplatin-treated human kidney-2 cells (HK-2 cells) when compared to hASCs modified by empty-vector. Additionally, hASCs modified by empty-vector inhibited caspase-3 expression and up-regulated Bcl-2 expression in cisplatin-treated HK-2 cells, an effect even more pronounced with hASCs modified by HIF-1α. Thus, HIF-1α gene-modified ASCs could be an effective way to enhance the renal-protective effect.

Keywords

Acute kidney injury Cisplatin Human adipose-derived stem cells Human kidney-2 cells Hypoxia inducible factor-1α Renal injury 

Supplementary material

10529_2013_1389_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • WeiWei Wang
    • 1
  • Wei Wang
    • 2
  • Yan Jiang
    • 2
  • Zezheng Li
    • 2
  • Jin Cheng
    • 1
  • Nanmei Liu
    • 1
  • GuoFeng Han
    • 1
  • Shi Lu
    • 1
  • JinYuan Zhang
    • 1
  1. 1.Department of NephrologyJimin HospitalShanghaiPeople’s Republic of China
  2. 2.Graduate SchoolShanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China

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