Biotechnology Letters

, Volume 36, Issue 3, pp 531–536

Production of fructose from highly concentrated date extracts using Saccharomyces cerevisiae

  • Meilana Dharma Putra
  • A. E. Abasaeed
  • S. M. Al-Zahrani
  • M. H. Gaily
  • A. K. Sulieman
  • M. A. Zeinelabdeen
  • H. K. Atiyeh
Original Research Paper

Abstract

Large amounts of low-quality dates produced worldwide are wasted. Here, highly concentrated fructose syrups were produced via selective fermentation of date extracts with Saccharomyces cerevisiae. Syrups with 95.4–99.9 % (w/w) fructose yields were obtained from date extracts having an initial sugar range of 49–374 g/l without media supplementation; the corresponding ethanol yields were between 69 and 52 % (w/w). At 470 g initial sugars/l, fructose and ethanol yields were 84 and 47 % (w/w), respectively, and the product contained 62 % (w/w) fructose, which is higher than the widely available commercial 42 and 55 % (w/w) high fructose corn syrups. The commercial potential for conversion of waste dates to high-value products is thus demonstrated.

Keywords

Dates Ethanol Fructose High fructose syrup Saccharomyces cerevisiae Selective fermentation 

References

  1. Abasaeed AE, Lee YY (1995) Inulin hydrolysis to fructose by a novel catalyst. Chem Eng Technol 18:440–444CrossRefGoogle Scholar
  2. Atiyeh H, Duvnjak Z (2001) Production of fructose and ethanol from media with high sucrose concentrations by a mutant of Saccharomyces cerevisiae. J Chem Tech Biotechnol 76:1017–1022CrossRefGoogle Scholar
  3. Atiyeh H, Duvnjak Z (2002) Production of fructose and ethanol from sugar beet molasses using Saccharomyces cerevisiae ATCC 36858. Biotechnol Prog 18:234–239PubMedCrossRefGoogle Scholar
  4. Baliga MS, Baliga BRV, Kandathil SM, Bhat HP, Vayalil PK (2011) A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Int 44:1812–1822CrossRefGoogle Scholar
  5. Bringer-Meyer S, Scollar M, Sahm H (1985) Zymomonas mobilis mutants blocked in fructose utilization. Appl Microbiol Biotechnol 23:134–139CrossRefGoogle Scholar
  6. Carvalho R, Gomes L, Gonzaga do P. Filho L, Tavares FA (2008) Obtaining and selection of hexokinases-less strains of Saccharomyces cerevisiae for production of ethanol and fructose from sucrose. Appl Microbiol Biotechnol 77:1131–1137PubMedCrossRefGoogle Scholar
  7. Doelle M, Doelle H (1991) High fructose formation from sugarcane syrup and molasses using Zymomonas mobilis mutants. Biotechnol Lett 13:875–878CrossRefGoogle Scholar
  8. Doelle H, Greenfield P (1985) Fermentation pattern of Zymomonas mobilis at high sucrose concentrations. Appl Microbiol Biotechnol 22:411–415Google Scholar
  9. Gaily MH, Elhassan BM, Abasaeed AE, Al-Shrhan M (2010) Isomerization and kinetics of glucose into fructose. Int J Eng Technol 3:1–10Google Scholar
  10. Jain SM (2012) In vitro mutagenesis for improving date palm (Phoenix dactylifera L.). Emir J Food Agric 24:400–407Google Scholar
  11. Johnson R, Padmaja G, Moorthy SN (2009) Comparative production of glucose and high fructose syrup from cassava and sweet potato roots by direct conversion techniques. Innov Food Sci Emerg Technol 10:616–620CrossRefGoogle Scholar
  12. Jones RP, Pamment N, Greenfield PF (1981) Alcohol fermentation by yeasts—the effect of environmental and other variables. Process Biochem 16:42–49Google Scholar
  13. Koren DW, Duvnjak Z (1992) Fed-batch production of concentrated fructose syrup and ethanol using Saccharomyces cerevisiae ATCC 36859. Acta Biotechnol 12:489–496CrossRefGoogle Scholar
  14. Lee KJ, Tribe DE, Rogers PL (1979) Ethanol production by Zymomonas mobilis in continous culture at high glucose concentrations. Biotechnol Lett 1:421–426CrossRefGoogle Scholar
  15. Maiorella BL, Blanch HW, Wilke CR (1984) Feed component inhibition in ethanolic fermentation by Saccharomyces cerevisiae. Biotechnol Bioeng 26:1155–1166PubMedCrossRefGoogle Scholar
  16. Moshaf S, Hamidi-Esfahani Z, Azizi MH (2011) Optimization of conditions for xanthan gum production from waste date in submerged fermantation. World Acad Sci Eng Technol 57:521–524Google Scholar
  17. Nuanpeng S, Laopaiboon L, Srinophakun P, Klanrit P, Jaisil P, Laopaiboon P (2011) Ethanol production from sweet sorghum juice under very high gravity conditions: batch, repeated-batch and scale up fermentation. Electron J Biotechnol 14:1–12Google Scholar
  18. Oura E (1977) Reaction products of yeast fermentations. Process Biochem 12:19–21Google Scholar
  19. Paugam MF, Riggs JA, Smith BD (1996) High fructose syrup production using fructose-selective liquid membranes. Chem Commun 22:2539–2540CrossRefGoogle Scholar
  20. Stewart GG, Russell I (1987) Biochemical and genetic control of sugar and carbohydrate metabolism in yeasts. Yeast biotechnology. Allen & Unwin, LondonGoogle Scholar
  21. Suntinanalert P, Pemberton J, Doelle H (1986) The production of ethanol plus fructose sweetener using fructose utilization negative mutants of Zymomonas mobilis. Biotechnol Lett 8:351–356CrossRefGoogle Scholar
  22. Zhang Y, Hidajat K, Ray AK (2004) Optimal design and operation of SMB bioreactor: production of high fructose syrup by isomerization of glucose. Biochem Eng J 21:111–121CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Meilana Dharma Putra
    • 1
  • A. E. Abasaeed
    • 1
  • S. M. Al-Zahrani
    • 1
  • M. H. Gaily
    • 1
  • A. K. Sulieman
    • 1
  • M. A. Zeinelabdeen
    • 1
  • H. K. Atiyeh
    • 2
  1. 1.Department of Chemical Engineering, College of EngineeringKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Biosystems and Agricultural EngineeringOklahoma State UniversityStillwaterUSA

Personalised recommendations