Biotechnology Letters

, Volume 36, Issue 3, pp 497–505 | Cite as

Comparison of extraction phases for a two-phase culture of a recombinant E. coli producing retinoids

  • Hui-Jeong Jang
  • Bo-Kyung Ha
  • Jin-Woong Kim
  • Kyung-Hwa Jung
  • Jiyoon Ahn
  • Sang-Hwal YoonEmail author
  • Seon-Won Kim
Original Research Paper


To prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the highest retinoid production as 134 mg/l after 72 h. For mineral oil, light and heavy mineral oil gave retinoid productions of 158 and 174 mg/l after 96 h, respectively. Of other materials, isopropyl myristate gave the highest retinoid production of 181 mg/l. These results indicate that many types of oils can be applied for retinoid production, and optimization of the in situ extraction process will lead to further improve of economical production for the industrial purpose.


Escherichia coli In situ extraction Retinoids Two-phase culture 



This work was supported by a grant (NRF-2010-C1AAA001-0029084) from the National Research Foundation, the Intelligent Synthetic Biology Center of Global Frontier Project funded by the MEST (2011-0031964), and a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant#: PJ009522003), RDA, Korea.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hui-Jeong Jang
    • 1
  • Bo-Kyung Ha
    • 1
  • Jin-Woong Kim
    • 2
  • Kyung-Hwa Jung
    • 3
  • Jiyoon Ahn
    • 4
  • Sang-Hwal Yoon
    • 1
    Email author
  • Seon-Won Kim
    • 1
  1. 1.Division of Applied Life Science (BK21 Program), PMBBRCGyeongsang National UniversityJinjuKorea
  2. 2.Department of Applied ChemistryHanyang UniversityAnsanKorea
  3. 3.Department of Food Science & TechnologyKyungsung UniversityPusanKorea
  4. 4.Department of Life SciencesImperial College LondonLondonUK

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