Lectin of Abelmoschus esculentus (okra) promotes selective antitumor effects in human breast cancer cells
- 1.1k Downloads
The anti-tumor effects of a newly-discovered lectin, isolated from okra, Abelmoschus esculentus (AEL), were investigated in human breast cancer (MCF7) and skin fibroblast (CCD-1059 sk) cells. AEL induced significant cell growth inhibition (63 %) in MCF7 cells. The expression of pro-apoptotic caspase-3, caspase-9, and p21 genes was increased in MCF7 cells treated with AEL, compared to those treated with controls. In addition, AEL treatment increased the Bax/Bcl-2 ratio in MCF7 cells. Flow cytometry also indicated that cell death (72 %) predominantly occurred through apoptosis. Thus, AEL in its native form promotes selective antitumor effects in human breast cancer cells and may represent a potential therapeutic to combat human breast cancer.
KeywordsAbelmoschus esculentus Apoptosis Cancer inhibition Human breast cancer Lectin Okra Plants
This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Grants no 02841/09-6 and 23.038.019120/9-3, Fundação de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS), Grants no. 11/1842-5 and Conselho Nacional de Pesquisa (CNPq). The authors also wish to thank Dr. Cláudia Pinho Hartleben, MSc. Eduarda Schultze, MSc. Karine Rech Begnini and MSc. Virginia Campello Yurgel for their valuable technical assistance with the flow cytometric analysis.
- Begnini KR, Rizzi C, Campos VF, Borsuk S, Schultze E, Yurgel VC, Nedel F, Dellagostin OA, Collares T, Seixas FK (2013) Auxotrophic recombinant Mycobacterium bovis BCG overexpressing Ag85B enhances cytotoxicity on superficial bladder cancer cells in vitro. Appl Microbiol Biotechnol 97:1543–1552PubMedCrossRefGoogle Scholar
- Campos VF, Collares T, Deschamps JC, Seixas FK, Dellagostin OA, Lanes CF, Sandrini J, Marins LF, Okamoto M, Sampaio LA, Robaldo RB (2010) Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus. J Biosci 35:405–413PubMedCrossRefGoogle Scholar
- Kim MS, Lee J, So HS, Lee KM, Jung BH, Chung SY, Moon SR, Kim NS, Ko CB, Kim HJ, Kim YK, Park R (2001) Gamma-interferon (IFN-gamma) augments apoptotic response to mistletoe lectin-II via upregulation of Fas/Fas L expression and caspase activation in human myeloid U937 cells. Immunopharmacol Immunotoxicol 23:55–66PubMedCrossRefGoogle Scholar