Biotechnology Letters

, Volume 36, Issue 1, pp 167–173 | Cite as

Molecular cloning and expression of a novel laccase showing thermo- and acid-stability from the endophytic fungus Phomopsis liquidambari and its potential for growth promotion of plants

  • Hong-Wei Wang
  • Hong Zhu
  • Xue-Fei Liang
  • Wei Du
  • Chuan-Chao Dai
Original Research Paper
First Online:
Received:
Accepted:

Abstract

A novel laccase (LACB3) from the endophytic fungus, Phomopsis liquidambari, was cloned and its potential to promote peanut growth was evaluated. The full-length cDNA is 1,731 bp, encoding a mature protein of 556 amino acids with a molecular mass of 60.1 kDa. Using 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate), LACB3 exhibited a K m and k cat of 85 μM and 92.7 s−1, respectively. The enzyme had maximal activity at pH 2.5 and 50 °C and retained 50 % of its activity after 20 h at 50 °C. When LACB3 was applied to soil, the peanut biomass was increased by 12 %, and the content of vanillic acid, coumaric acid and 4-hydroxybenzoic acid in soil were decreased by 21, 27 and 40 %, respectively. These results suggest substantial potential for the use of P. liquidambari or its laccase in agriculture.

Keywords

Endophyte Laccase Peanut biomass Phomopsis liquidambari Plant growth promotion 
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Notes

Acknowledgments

We thank Prof. Ying Huang (Nanjing Normal University) for technical assistance. We are grateful to the National Natural Science Foundation of China (NSFC NOs. 31370507, 31070443, 30970523), the key project for Natural Science Foundation of Jiangsu Higher Education Institutions (13KJA180003) for their financial support.

Supplementary material

10529_2013_1347_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hong-Wei Wang
    • 1
  • Hong Zhu
    • 1
  • Xue-Fei Liang
    • 1
  • Wei Du
    • 1
  • Chuan-Chao Dai
    • 1
  1. 1.Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life SciencesNanjing Normal UniversityNanjingChina

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