Advertisement

Biotechnology Letters

, Volume 35, Issue 11, pp 1831–1838 | Cite as

Proteome profile of bovine ruminal epithelial tissue based on GeLC–MS/MS

  • Yongxin Yang
  • Jiaqi WangEmail author
  • Tingjie Yuan
  • Dengpan Bu
  • Jinhui Yang
  • Peng Sun
Original Research Paper

Abstract

The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC–MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities related to their molecular functions, were proteins of cellular components or belonged to various metabolic processes. A predominant group of canonical pathways in the rumen epithelial tissue was identified using the IPA software. The GeLC–MS/MS approach was used to characterise the entire protein expression repertoire in rumen tissue, providing a more detailed understanding of the important biological processes in the rumen.

Keywords

Animal proteomics Cow GeLC–MS/MS Proteomic analysis Ruminal epithelial tissue 

Notes

Acknowledgments

The project was supported by the National Key Basic Research Program of China (Project No. 2011CB100805).

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

10529_2013_1291_MOESM1_ESM.xls (3.3 mb)
Supplementary material 1 (XLS 3340 kb)

References

  1. Abdoun K, Stumpff F, Martens H (2006) Ammonia and urea transport across the rumen epithelium: a review. Anim Health Res Rev 7:43–59PubMedCrossRefGoogle Scholar
  2. Bendixen E, Danielsen M, Hollung K, Gianazza E, Miller I (2011) Farm animal proteomics–a review. J Proteomics 74:282–293PubMedCrossRefGoogle Scholar
  3. Bondzio A, Gabler C, Badewien-Rentzsch B, Schulze P, Martens H, Einspanier R (2011) Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet. Am J Physiol Gastrointest Liver Physiol 301:G260–268PubMedCrossRefGoogle Scholar
  4. Fu-Jun L, Hai-Yan W, Jian-Yuan L (2012) A new analysis of testicular proteins through integrative bioinformatics. Mol Biol Rep 39:3965–3970PubMedCrossRefGoogle Scholar
  5. Gerling IC, Singh S, Lenchik NI, Marshall DR, Wu J (2006) New data analysis and mining approaches identify unique proteome and transcriptome markers of susceptibility to autoimmune diabetes. Mol Cell Proteomics 5:293–305PubMedGoogle Scholar
  6. Klopfleisch R, Gruber AD (2012) Transcriptome and proteome research in veterinary science: what is possible and what questions can be asked? ScientificWorldJournal 2012:254962PubMedCrossRefGoogle Scholar
  7. Krishnan S, Gaspari M, Della Corte A, Bianchi P, Crescente M, Cerletti C, Torella D, Indolfi C, de Gaetano G, Donati MB, Rotilio D, Cuda G (2011) OFFgel-based multidimensional LC–MS/MS approach to the cataloguing of the human platelet proteome for an interactomic profile. Electrophoresis 32:686–695PubMedCrossRefGoogle Scholar
  8. Krogh A, Larsson B, von Heijne G, Sonnhammer EL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580PubMedCrossRefGoogle Scholar
  9. Nie L, Wu G, Culley DE, Scholten JC, Zhang W (2007) Integrative analysis of transcriptomic and proteomic data: challenges, solutions and applications. Crit Rev Biotechnol 27:63–75PubMedCrossRefGoogle Scholar
  10. Peddinti D, Memili E, Burgess SC (2010) Proteomics-based systems biology modeling of bovine germinal vesicle stage oocyte and cumulus cell interaction. PLoS ONE 5:e11240PubMedCrossRefGoogle Scholar
  11. Petersen TN, Brunak S, von Heijne G, Nielsen H (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 8:785–786PubMedCrossRefGoogle Scholar
  12. Pisanu S, Ghisaura S, Pagnozzi D, Biosa G, Tanca A, Roggio T, Uzzau S, Addis MF (2011) The sheep milk fat globule membrane proteome. J Proteomics 74:350–358PubMedCrossRefGoogle Scholar
  13. Schirle M, Heurtier MA, Kuster B (2003) Profiling core proteomes of human cell lines by one-dimensional PAGE and liquid chromatography-tandem mass spectrometry. Mol Cell Proteomics 2:1297–1305PubMedCrossRefGoogle Scholar
  14. Sehested J, Diernaes L, Moller PD, Skadhauge E (1999) Transport of butyrate across the isolated bovine rumen epithelium–interaction with sodium, chloride and bicarbonate. Comp Biochem Physiol A Mol Integr Physiol 123:399–408PubMedCrossRefGoogle Scholar
  15. Steele MA, Croom J, Kahler M, Alzahal O, Hook SE, Plaizier K, McBride BW (2011) Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis. Am J Physiol Regul Integr Comp Physiol 300:R1515–1523PubMedCrossRefGoogle Scholar
  16. Taniguchi M, Penner GB, Beauchemin KA, Oba M, Guan LL (2010) Comparative analysis of gene expression profiles in ruminal tissue from Holstein dairy cows fed high or low concentrate diets. Comp Biochem Physiol Part D Genomics Proteomics 5:274–279PubMedCrossRefGoogle Scholar
  17. Torres-Garcia W, Zhang W, Runger GC, Johnson RH, Meldrum DR (2009) Integrative analysis of transcriptomic and proteomic data of Desulfovibrio vulgaris: a non-linear model to predict abundance of undetected proteins. Bioinformatics 25:1905–1914PubMedCrossRefGoogle Scholar
  18. Wang Z, Hill S, Luther JM, Hachey DL, Schey KL (2012) Proteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT). Proteomics 12:329–338PubMedCrossRefGoogle Scholar
  19. Yousuf MA, Mi-ichi F, Nakada-Tsukui K, Nozaki T (2010) Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica. Eukaryot Cell 9:926–933PubMedCrossRefGoogle Scholar
  20. Zhou L, Zhao SZ, Koh SK, Chen L, Vaz C, Tanavde V, Li XR, Beuerman RW (2012) In-depth analysis of the human tear proteome. J Proteomics 75:3877–3885PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yongxin Yang
    • 1
    • 2
  • Jiaqi Wang
    • 1
    Email author
  • Tingjie Yuan
    • 1
  • Dengpan Bu
    • 1
  • Jinhui Yang
    • 1
  • Peng Sun
    • 1
  1. 1.State Key Laboratory of Animal Nutrition, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Institute of Animal Science and Veterinary MedicineAnhui Academy of Agricultural SciencesHefeiChina

Personalised recommendations