Proteome profile of bovine ruminal epithelial tissue based on GeLC–MS/MS
- 346 Downloads
The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC–MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities related to their molecular functions, were proteins of cellular components or belonged to various metabolic processes. A predominant group of canonical pathways in the rumen epithelial tissue was identified using the IPA software. The GeLC–MS/MS approach was used to characterise the entire protein expression repertoire in rumen tissue, providing a more detailed understanding of the important biological processes in the rumen.
KeywordsAnimal proteomics Cow GeLC–MS/MS Proteomic analysis Ruminal epithelial tissue
The project was supported by the National Key Basic Research Program of China (Project No. 2011CB100805).
Conflict of interest
The authors have declared no conflict of interest.
- Krishnan S, Gaspari M, Della Corte A, Bianchi P, Crescente M, Cerletti C, Torella D, Indolfi C, de Gaetano G, Donati MB, Rotilio D, Cuda G (2011) OFFgel-based multidimensional LC–MS/MS approach to the cataloguing of the human platelet proteome for an interactomic profile. Electrophoresis 32:686–695PubMedCrossRefGoogle Scholar