Biotechnology Letters

, Volume 35, Issue 11, pp 1831–1838 | Cite as

Proteome profile of bovine ruminal epithelial tissue based on GeLC–MS/MS

  • Yongxin Yang
  • Jiaqi WangEmail author
  • Tingjie Yuan
  • Dengpan Bu
  • Jinhui Yang
  • Peng Sun
Original Research Paper


The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC–MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities related to their molecular functions, were proteins of cellular components or belonged to various metabolic processes. A predominant group of canonical pathways in the rumen epithelial tissue was identified using the IPA software. The GeLC–MS/MS approach was used to characterise the entire protein expression repertoire in rumen tissue, providing a more detailed understanding of the important biological processes in the rumen.


Animal proteomics Cow GeLC–MS/MS Proteomic analysis Ruminal epithelial tissue 



The project was supported by the National Key Basic Research Program of China (Project No. 2011CB100805).

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

10529_2013_1291_MOESM1_ESM.xls (3.3 mb)
Supplementary material 1 (XLS 3340 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yongxin Yang
    • 1
    • 2
  • Jiaqi Wang
    • 1
    Email author
  • Tingjie Yuan
    • 1
  • Dengpan Bu
    • 1
  • Jinhui Yang
    • 1
  • Peng Sun
    • 1
  1. 1.State Key Laboratory of Animal Nutrition, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Institute of Animal Science and Veterinary MedicineAnhui Academy of Agricultural SciencesHefeiChina

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