Biotechnology Letters

, Volume 35, Issue 11, pp 1945–1952

RNA-binding proteins associated with desiccation during seed development in rice

  • Naoto Sano
  • Shunpei Masaki
  • Takanari Tanabata
  • Tetsuya Yamada
  • Tadashi Hirasawa
  • Megumi Kashiwagi
  • Motoki Kanekatsu
Original Research Paper

DOI: 10.1007/s10529-013-1283-6

Cite this article as:
Sano, N., Masaki, S., Tanabata, T. et al. Biotechnol Lett (2013) 35: 1945. doi:10.1007/s10529-013-1283-6

Abstract

Long-lived mRNAs stored in mature seeds can remain active for long periods even if seeds undergo severe desiccation. They are then translated at the initiation of germination. To clarify the mechanism for stabilization of long-lived mRNAs during seed desiccation, fluctuations in RNA-binding protein (RBP) profiles that occur during seed formation in rice were analyzed. Proteomic analysis revealed that glycine-rich RBP 1A (GRP1A) is a highly abundant RBP in mature rice seeds. In addition, real-time RT-PCR analysis showed that putative RBP RZ-1A (RZ-1A) is seed specific. Moreover, transcripts of these two RBPs were clearly up-regulated during desiccation in rice seeds. The features of these two RBPs resemble those of late embryogenesis abundant proteins that function as molecular chaperones in dry seeds. Therefore, GRP1A and RZ-1A may have important roles in the stability of long-lived mRNAs in rice seeds.

Keywords

Late embryogenesis abundant protein Long-lived mRNA Proteome Rice RNA-binding protein Seed desiccation 

Supplementary material

10529_2013_1283_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 17 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Naoto Sano
    • 1
    • 2
  • Shunpei Masaki
    • 1
  • Takanari Tanabata
    • 3
  • Tetsuya Yamada
    • 1
  • Tadashi Hirasawa
    • 1
  • Megumi Kashiwagi
    • 1
  • Motoki Kanekatsu
    • 1
  1. 1.Department of Plant Production, United Graduate School of Agricultural ScienceTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.Japan Society for the Promotion of ScienceChiyodaJapan
  3. 3.Gene Discovery Research GroupRIKEN Center for Sustainable Resource ScienceTsukubaJapan

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