Biotechnology Letters

, Volume 35, Issue 11, pp 1839–1843 | Cite as

Differential sensitivities of the growth of Escherichia coli to acrylate under aerobic and anaerobic conditions and its effect on product formation

Original Research Paper

Abstract

The effect of acrylate on the growth of Escherichia coli was determined under aerobic and anaerobic conditions in glucose-defined medium. Growth occurred with up to 35 mM acrylate under aerobic conditions but ceased at 5 mM acrylate under anaerobic conditions. This differential sensitivity can be attributed to inhibition of pyruvate formate lyase and/or pflB gene repression, as this enzyme is necessary for anaerobic growth of E. coli. The effect of acrylate on end-product distribution was also determined by growing E. coli first aerobically, then switching to anaerobic conditions. In the absence of acrylate, E. coli generated the typical distribution of mixed-acid products, with about 12 % of pyruvate being metabolically converted to lactate. In contrast, in the presence of 5 mM acrylate, E. coli converted 83 % of pyruvate to lactate, consistent with a reduction in pyruvate formate lyase activity.

Keywords

Acrylate Aerobic/anaerobic effects E. coli Lactate Pyruvate formate lyase Pyruvate metabolism 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Ajay S. Arya
    • 1
    • 2
  • Sarah A. Lee
    • 1
  • Mark A. Eiteman
    • 1
    • 2
  1. 1.BioChemical Engineering, College of EngineeringUniversity of GeorgiaAthensUSA
  2. 2.Department of MicrobiologyUniversity of GeorgiaAthensUSA

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