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Biotechnology Letters

, Volume 35, Issue 11, pp 1807–1815 | Cite as

3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells

  • En-Ji Cui
  • Jeon Hwang-Bo
  • Jong-Hwa Park
  • Nam-In Baek
  • Jiyoung Kim
  • Seong Gil Hong
  • In Sik Chung
Original Research Paper

Abstract

3-O-Acetyloleanolic acid, a pentacyclic triterpenoid isolated from cowpea seeds, inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. HUVECs. The induced apoptosis was characterized by detection of cell surface annexin V and sub-G1 populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell populations were also increased in treated HUVECs. 3-O-Acetyloleanolic acid induced activation of caspase 3, a critical mediator of apoptosis signaling. It also significantly inhibited angiogenesis in an in vivo Matrigel plug assay. 3-O-Acetyloleanolic acid thus exhibits anti-angiogenic effects and induces apoptosis in HUVECs and the results suggest that it has a potential use for suppression of the tumor growth stimulated by angiogenesis.

Keywords

3-O-Acetyloleanolic acid Apoptosis Human umbilical vein endothelial cell Matrigel plug assay 

Notes

Acknowledgments

This study was supported by a Grant from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1B3001575).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • En-Ji Cui
    • 1
    • 2
  • Jeon Hwang-Bo
    • 1
    • 2
  • Jong-Hwa Park
    • 1
    • 2
  • Nam-In Baek
    • 2
  • Jiyoung Kim
    • 2
  • Seong Gil Hong
    • 3
  • In Sik Chung
    • 1
    • 2
    • 4
  1. 1.Department of Genetic EngineeringKyung Hee UniversityYonginKorea
  2. 2.Graduate School of BiotechnologyKyung Hee UniversityYonginKorea
  3. 3.Erom Life Co. Ltd.SeoulKorea
  4. 4.Department of Genetic Engineering and Graduate School of BiotechnologyKyung Hee UniversityYonginKorea

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