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3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells

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Abstract

3-O-Acetyloleanolic acid, a pentacyclic triterpenoid isolated from cowpea seeds, inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. HUVECs. The induced apoptosis was characterized by detection of cell surface annexin V and sub-G1 populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell populations were also increased in treated HUVECs. 3-O-Acetyloleanolic acid induced activation of caspase 3, a critical mediator of apoptosis signaling. It also significantly inhibited angiogenesis in an in vivo Matrigel plug assay. 3-O-Acetyloleanolic acid thus exhibits anti-angiogenic effects and induces apoptosis in HUVECs and the results suggest that it has a potential use for suppression of the tumor growth stimulated by angiogenesis.

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Acknowledgments

This study was supported by a Grant from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1B3001575).

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Authors and Affiliations

  1. Department of Genetic Engineering, Kyung Hee University, Yongin, 446-701, Korea

    En-Ji Cui, Jeon Hwang-Bo, Jong-Hwa Park & In Sik Chung

  2. Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    En-Ji Cui, Jeon Hwang-Bo, Jong-Hwa Park, Nam-In Baek, Jiyoung Kim & In Sik Chung

  3. Erom Life Co. Ltd., Seoul, 135-825, Korea

    Seong Gil Hong

  4. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    In Sik Chung

Authors
  1. En-Ji Cui
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  2. Jeon Hwang-Bo
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  3. Jong-Hwa Park
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  4. Nam-In Baek
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  5. Jiyoung Kim
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  6. Seong Gil Hong
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  7. In Sik Chung
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Corresponding author

Correspondence to In Sik Chung.

Additional information

En-Ji Cui and Jeon Hwang-Bo have contributed equally to this study.

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Cui, EJ., Hwang-Bo, J., Park, JH. et al. 3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells. Biotechnol Lett 35, 1807–1815 (2013). https://doi.org/10.1007/s10529-013-1266-7

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  • DOI: https://doi.org/10.1007/s10529-013-1266-7

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