Development of a chloroplast DNA marker for monitoring of transgene introgression in Brassica napus L.
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Chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in Brassicaceae and for differentiation between its species. Combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (GM) plants through hybridization studies. To develop chloroplast DNA markers for monitoring of transgene introgression in Brassica napus L., we searched for sequence variations in the chloroplast (cp) genome, and developed a simple cpDNA marker that is reliable, time-saving, and easily discriminates among 4 species (B. napus, B. rapa, Raphanus sativus, and Sinapis alba) based on PCR-product length polymorphism. This marker will be useful to identify maternal lineages and to estimate transgene movement of GM canola.
KeywordsBrassica napus Chloroplast DNA marker cpDNA Intergenic region Simple PCR
This study was supported by a grant from Research Program for Agricultural Science & Technology Development (Project No. PJ008545), National Academy of Agricultural Science and the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ008021), Rural Development Administration, Republic of Korea.
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